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| Effects of UV-B radiation on the expression of four pathogenic genes in the infection stage of Magnaporthe grisea |
| Received:May 12, 2018 |
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| KeyWord:UV-B;real-time PCR;pathogenicity;rice blast;infection process |
| Author Name | Affiliation | E-mail | | HUANG Lan-lin | College of Resources and Environment, Yunnan Agricultural University, Kunming 650201, China | | | MEI Xin-yue | College of Resources and Environment, Yunnan Agricultural University, Kunming 650201, China | | | LI Xiang | College of Resources and Environment, Yunnan Agricultural University, Kunming 650201, China | | | LI Xiang | College of Resources and Environment, Yunnan Agricultural University, Kunming 650201, China | | | ZU Yan-qun | College of Resources and Environment, Yunnan Agricultural University, Kunming 650201, China | | | HE Yong-mei | College of Resources and Environment, Yunnan Agricultural University, Kunming 650201, China | | | LI Yuan | College of Resources and Environment, Yunnan Agricultural University, Kunming 650201, China | liyuan@ynau.edu.cn |
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| Abstract: |
| Rice blast is a serious disease affecting rice. Four pathogenic genes in rice blast fungi were used to study the molecular pathogenicity mechanism of rice blast treated by UV-B radiation. Ultraviolet light to imitate emittances of 0, 2.5 and 5 kJ·m-2 to the rice blast and real-time PCR were used to analyze the expression of four pathogenicity-related genes (Chitinase, MGP1, MAGB, CPKA). The results showed that there was no significant change in the expression of MGP1 with an increase in the UV-B radiation dose, while the expressions of Chitinase, MAGB, and CPKA were down-regulated. After removing UV-B, expressions rebounded after 6 and 24 h. The expression of Chitinase and CPKA was greatly affected by a UV-B intensity of 5 kJ·m-2, and it was down-regulated by 94% and 61%, particularly with a radiation duration of 120 min. The change in the expression of MAGB expression was greater under 2.5 kJ·m-2, and was down-regulated by 57%, 17% and 40% from the beginning to the end of radiation. According to the dyeing experiment at the infection stage, UV-B could effectively inhibit the germination and growth of the germ tube, appressorium, and hyphae, which were 45.1%, 82.2% and 75.2% of those of the control at a radiation of 5 kJ·m-2, respectively. Overall, enhanced UV-B radiation can down-regulate the expression of these diseasecausing genes and reduce and inhibit the growth of Magnaporthe grisea in the infection stage, thus affecting its pathogenicity. Finally, the effect of a radiation intensity of 5 kJ·m-2 is more significant. |
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