|
| Dynamics of heavy metal residues, antibiotic resistance genes, and bacterial communities during chicken manure composting |
| Received:May 30, 2018 Revised:July 25, 2018 |
| View Full Text View/Add Comment Download reader |
| KeyWord:chicken manure;composting;bacterial community;heavy metal;antibiotic resistance gene |
| Author Name | Affiliation | E-mail | | DENG Wen-wen | College of Resources, Sichuan Agricultural University, Chengdu 611130, China | | | CHEN Shu-juan | College of Food Science, Sichuan Agricultural University, Ya′an 625014, China | | | HE Xue-ping | College of Food Science, Sichuan Agricultural University, Ya′an 625014, China | | | JIN Lei | College of Resources, Sichuan Agricultural University, Chengdu 611130, China | | | YANG Sheng-zhi | College of Resources, Sichuan Agricultural University, Chengdu 611130, China | | | YU Xiu-mei | College of Resources, Sichuan Agricultural University, Chengdu 611130, China | | | LIU Shu-liang | College of Food Science, Sichuan Agricultural University, Ya′an 625014, China | | | ZOU Li-kou | College of Resources, Sichuan Agricultural University, Chengdu 611130, China | zoulikou@sicau.edu.cn |
|
| Hits: 2075 |
| Download times: 2306 |
| Abstract: |
| The changes in the content of nutrients and heavy metals, abundance of antibiotic resistance genes (ARGs), and the succession law of bacterial communities were investigated during chicken manure composting to explain the correlations between bacterial communities and nutrients, heavy metals, and ARGs. Fresh chicken manure (FM)and its compost samples (FC and SC)were collected, and their nutrient and heavy metal content, and the abundance of ARGs were determined. The succession law of bacterial community was analyzed by 16S rRNA high-throughput sequencing. After composting, the content of organic matter decreased, but the content of total nitrogen and phosphorus increased at different levels, whereas, the total potassium content and pH significantly increased (P<0.05). The heavy metal content fluctuated in different sample groups. Compared with those in FM, the concentration of arsenic and cadmium increased in SC,whereas the content of copper, manganese, lead, and zinc (Zn)decreased in SC; only the concentration of Zn decreased significantly (P< 0.05). The abundance of the ARGs aac (6')-Ib-cr, tetM (P<0.05), ermB (P<0.05), and blaCTX-M decreased (P<0.05)at different levels after composting, but the abundance of the gene sul1 increased. The results showed that the phyla Firmicutes, Proteobacteria, and Bacteroidetes were dominant in all the groups. However, each group had different dominant species at the genus level, and the abundance of potential pathogens reduced during composting. The correlation analysis revealed that the most dominant genera significantly correlated with the content of total phosphorus and Cr, pH, and abundance of the ARGs sul1, tetM, ermB, and blaCTX-M (P<0.05). The relative abundance of pathogens and ARGs can decrease after composting, but there are still some risks. This study provides a reference for efficient and safe composting treatment of chicken manure during actual production. |
|
|
|
