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| Effects of continuous planting of transgenic cotton on the community structure of AM fungi in soil |
| Received:March 20, 2018 Revised:July 09, 2018 |
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| KeyWord:arbuscular mycorrhizal fungi;transgenic cotton;Polymerase chain reaction-Denaturing gradient gel electrophoresis (PCR-DGGE) |
| Author Name | Affiliation | E-mail | | LIU Rui-hua | College of Agronomy & Resources and Environment, Tianjin Agricultural University, Tianjin 300384, China
Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China
Key Laboratory of Original Agro-Environmental Pollution Prevention and Control, Ministry of Agriculture/Tianjin Key Laboratory of Agro-environment and Safe-product, Tianjin 300191, China | | | CHEN Jing-yi | College of Agronomy & Resources and Environment, Tianjin Agricultural University, Tianjin 300384, China
Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China
Key Laboratory of Original Agro-Environmental Pollution Prevention and Control, Ministry of Agriculture/Tianjin Key Laboratory of Agro-environment and Safe-product, Tianjin 300191, China | | | WANG Li-li | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China
Key Laboratory of Original Agro-Environmental Pollution Prevention and Control, Ministry of Agriculture/Tianjin Key Laboratory of Agro-environment and Safe-product, Tianjin 300191, China | | | LI Jing | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China
College of Resources and Environment, Northeast Agricultural University, Harbin, 150030, China
Key Laboratory of Original Agro-Environmental Pollution Prevention and Control, Ministry of Agriculture/Tianjin Key Laboratory of Agro-environment and Safe-product, Tianjin 300191, China | | | LIU Hui-fen | College of Agronomy & Resources and Environment, Tianjin Agricultural University, Tianjin 300384, China | | | YANG Dian-lin | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China
Key Laboratory of Original Agro-Environmental Pollution Prevention and Control, Ministry of Agriculture/Tianjin Key Laboratory of Agro-environment and Safe-product, Tianjin 300191, China | | | ZHAO Jian-ning | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China
Key Laboratory of Original Agro-Environmental Pollution Prevention and Control, Ministry of Agriculture/Tianjin Key Laboratory of Agro-environment and Safe-product, Tianjin 300191, China | zhaojn2008@163.com |
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| Abstract: |
| To detect the effects of cultivating transgenic crops on the community composition of soil arbuscular mycorrhizal (AM)fungi, we employed two transgenic cotton varieties, 013011 (drought-resistant)and SGK321 (insect-resistant), and their parental varieties TH2 (drought-resistant receptor)and Shiyuan321 (insect-resistant receptor). The Polymerase chain reaction-Denaturing gradient gel electrophoresis (PCR-DGGE)technology was used to analyze the community structure of the AM fungi in the soil. The results showed that the minimum similarity of the AM fungi in the soil with planted transgenic cotton and non-transgenic cotton was 0.6, indicating that the transgenic cotton planting had no significant effect on the community structure of the AM fungi in the soil during the blooming and the wadding stages. No significant difference in the richness (S)of the AM fungi was found between the soils planted with the transgenic and non-transgenic cotton during the blooming and wadding stages. The Shannon-Wiener index (H)of the AM fungi in the soil planted with Shiyuan 321 was significantly lower than that planted with SGK321 only at the wadding stage, However, no significant difference found among the other growing periods and other cotton types. The evenness (EH)of the AM fungi in the soil planted with TH2 was significantly higher than that planted with 013011 only at the wadding stage, but no significant difference was observed among the other cotton types and growing periods. The DGGE fingerprinting of the AM fungi in soil planted with the transgenic cotton was very similar to that planted with the non-transgenic cotton at the same growing stage, indicating that the impacts of planting the transgenic cotton on the community structure of the AM fungi in soil was small. The predominant genera were Glomus during both the flowering and boll-setting periods. The community structure of the AM fungi in the soil only change temporarily with the growing periods, but is hardly affected by the planting of the transgenic cotton. |
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