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| Oxidative stress response to MC-LR in the hepatopancreas of juvenile grass carp |
| Received:January 29, 2018 |
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| KeyWord:MC-LR;grass carp;hepatopancreas;antioxidation |
| Author Name | Affiliation | E-mail | | WEI Li-li | College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China | | | HE Li | College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China | | | RUAN Ji-ming | College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China | | | LIU Yi | College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China | | | FU Jian-ping | College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China | | | ZHONG Qi-wang | College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang 330045, China | fishimmuno@163.com,zhongqw2000@163.com |
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| Abstract: |
| To better understand the influence of microcystin-LR(MC-LR) on the oxidative stress response in the hepatopancreas of juvenile grass carp(Ctenopharyngodon idella), the present study was conducted injecting MC-LR intraperitoneally at doses of 25 μg MC-LR·kg-1 (low-dose group) and 100 μg MC-LR·kg-1 (high-dose group). Samples of hepatopancreases from juvenile grass carp were collected at 24, 48, and 72 h after the initial injection. The activities and transcriptional levels of antioxidant enzymes, including superoxide dismutase (SOD), catalase(CAT), glutathione peroxidase(GPx), and glutathione reductase(GR), were analyzed using spectrophotometry and quantitative real-time PCR, respectively. The enzymatic activity of SOD in the low-dose group was not significantly changed, but its content in the high-dose group was significantly enhanced at 24 and 48 h(P<0.05), while its content began to decrease at 72 h. The enzymatic activity of CAT just in the high-dose groups was significantly enhanced at 24 h(P<0.05), but the activities in the low-and high-dose groups all decreased at 48 h and 72 h with insignificant differences(P>0.05). The expressions of the SOD, CAT, and GPx genes were all significantly down-regulated during the whole experiment(P<0.05), but the expressions of the GR gene in the low-dose group were significantly up-regulated just at 24 h(P<0.05); another, its expression in two treated group were also up-regulated at 72 h, but the differences were not significant(P>0.05). However, the expressions of GR gene in the high dose group were all down-regulated with insignificant differences at 24 h and 48 h. In addition, correlation analysis of the enzymatic activities and gene expressions showed that the enzymatic activities of SOD and CAT were not correlated with their gene expressions. This study demonstrated that MC-LR could induce oxidative stress in juvenile grass carp by adjusting enzymatic activity and gene expression, but the mechanism of interaction between these needs to be further studied. |
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