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| Proteomics of butachlor-degrading bacterium Sphingobacterium thalpophilum under cadmium stress |
| Received:July 30, 2018 |
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| KeyWord:cadmium stress;butachlor;Sphingobacterium thalpophilum;2-D electrophoresis;proteomics |
| Author Name | Affiliation | E-mail | | LIN Xiao-yan | Rice Product Quality Supervision and Inspection Center, Ministry of Agriculture and Rural Affairs, China National Rice Research Institute, Hangzhou 310006, China
Laboratory of Quality & Safety Risk Assessment for Rice(Hangzhou), Ministry of Agriculture and Rural Affairs, Hangzhou 310006, China | | | MOU Ren-xiang | Rice Product Quality Supervision and Inspection Center, Ministry of Agriculture and Rural Affairs, China National Rice Research Institute, Hangzhou 310006, China
Laboratory of Quality & Safety Risk Assessment for Rice(Hangzhou), Ministry of Agriculture and Rural Affairs, Hangzhou 310006, China | | | CAO Zhao-yun | Rice Product Quality Supervision and Inspection Center, Ministry of Agriculture and Rural Affairs, China National Rice Research Institute, Hangzhou 310006, China
Laboratory of Quality & Safety Risk Assessment for Rice(Hangzhou), Ministry of Agriculture and Rural Affairs, Hangzhou 310006, China | | | CAO Zhen-zhen | Rice Product Quality Supervision and Inspection Center, Ministry of Agriculture and Rural Affairs, China National Rice Research Institute, Hangzhou 310006, China
Laboratory of Quality & Safety Risk Assessment for Rice(Hangzhou), Ministry of Agriculture and Rural Affairs, Hangzhou 310006, China | | | ZHOU Rong | Rice Product Quality Supervision and Inspection Center, Ministry of Agriculture and Rural Affairs, China National Rice Research Institute, Hangzhou 310006, China
Laboratory of Quality & Safety Risk Assessment for Rice(Hangzhou), Ministry of Agriculture and Rural Affairs, Hangzhou 310006, China | | | XU Ping | Rice Product Quality Supervision and Inspection Center, Ministry of Agriculture and Rural Affairs, China National Rice Research Institute, Hangzhou 310006, China
Laboratory of Quality & Safety Risk Assessment for Rice(Hangzhou), Ministry of Agriculture and Rural Affairs, Hangzhou 310006, China | | | CHEN Ming-xue | Rice Product Quality Supervision and Inspection Center, Ministry of Agriculture and Rural Affairs, China National Rice Research Institute, Hangzhou 310006, China
Laboratory of Quality & Safety Risk Assessment for Rice(Hangzhou), Ministry of Agriculture and Rural Affairs, Hangzhou 310006, China | cmingxue@126.com |
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| Abstract: |
| In order to determine the molecular mechanism of a highly efficient butachlor-degradation bacterium under cadmium stress at the protein expression level, the proteome change of Sphingobacterium thalpophilum sp. 15# was studied under cadmium stress. A 10 mg·L-1 cadmium stress treatment and a control without cadmium were employed. After seven days, the total protein of the strain from both the stress treatment and the control was sampled and extracted. Then, the differentially expressed proteins between the two treatments were analyzed by 2-D electrophoresis and mass spectrometry. A total of 93 differential protein spots were obtained, including 49 up-regulated protein spots and 44 down-regulated protein spots. 23 protein spots with good reproducibility and exhibiting the most obvious differences were further analyzed by MALDI-TOF-MS. 15 differential proteins were successfully identified, eight of which were up-regulated and seven of which were down-regulated. The identified proteins were annotated by bioinformatics of protein functions according to GO and COG. The proteins exhibiting significant differences were mainly those involved in the metabolism of butachlor, proteins that respond to cadmium stress, and proteins involved in the metabolism of butachlor under cadmium stress. Two cadmium-sensitive proteins (protein No. A0A0M7HIV1 and A0A0D6ⅡE6, respectively) and one protein that was resistant to cadmium stress and involved in butachlor metabolism (protein No. D4FMF4) were obtained. The obtained proteins are thought to play an important role in the degradation of butachlor under cadmium stress. |
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