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| Effects of Pseudomonas aeruginosa on subcellular distribution and chemical speciation of Cd in Solanum nigrum |
| Received:January 22, 2018 |
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| KeyWord:Pseudomonas aeruginosa;Solanum nigrum;Cd;subcellular distribution;chemical forms |
| Author Name | Affiliation | E-mail | | ZHANG Hai-li | School of Environment and Civil Engineering, Jiangnan University, Wuxi 214122, China | | | WANG Tao | School of Environment and Civil Engineering, Jiangnan University, Wuxi 214122, China | | | ZOU Lu-yi | School of Environment and Civil Engineering, Jiangnan University, Wuxi 214122, China | | | YU Hong-yan | School of Environment and Civil Engineering, Jiangnan University, Wuxi 214122, China | | | GU Wen-xiu | School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, China | | | TENG Yue | School of Environment and Civil Engineering, Jiangnan University, Wuxi 214122, China | tengyue@jiangnan.edu.cn |
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| Abstract: |
| The effects of Pseudomonas aeruginosa on the distribution and chemical forms of Cd in Solanum nigrum were studied via a potting experiment. The concentration of Cd in the pot experiment was set at 0, 25, 50, and 100 mg·kg-1. The subcellular distribution and chemical forms of Cd in Solanum nigrum were studied using differential centrifugation and stepwise extraction of chemical reagent. The results showed that the Cd content of Solanum nigrum leaves inoculated with Pseudomonas aeruginosa was 1.14~1.46 times higher than that of the non-inoculated leaves. Pseudomonas aeruginosa also promoted the transfer of Cd from roots to stems and leaves in the plants. Compared with the non-inoculation treatment, inoculation increased the Cd proportion in the cell wall of the roots and the Cd content in the soluble components of the leaves. In addition, inoculation decreased Cd uptake by up to 45.87% in the organelles of roots and leaves. Moreover, inoculation with Pseudomonas aeruginosa increased the proportion of low-activity Cd in the leaves of Solanum nigrum. Therefore, inoculation with Pseudomonas aeruginosa can promote the tolerance to Cd of Solanum nigrum by promoting the transfer of Cd to aboveground parts of the plant and changing the distribution and form ratio of Cd in the plant. These results will be beneficial for further studies on plant-microbe symbiosis in heavy-metal-contaminated soils. |
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