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| The response to the combined pollution of cadmium, zinc, and lead by the antioxidant enzyme activity of Lolium perenne L. |
| Received:October 31, 2017 |
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| KeyWord:heavy metals;orthogonal experiment;antioxidant enzyme;Lolium perenne L. |
| Author Name | Affiliation | | ZHANG Jun | Key Laboratory of Subsurface Hydrology and Ecological Effect in Arid Region of Ministry of Education, Chang'an University, Xi'an 710054, China
Shaanxi Key Laboratory of Disaster Monitoring and Mechanism Simulation, Baoji University of Arts and Sciences, Baoji 721013, China | | WANG Wen-ke | Key Laboratory of Subsurface Hydrology and Ecological Effect in Arid Region of Ministry of Education, Chang'an University, Xi'an 710054, China | | GENG Ya-ni | Shaanxi Key Laboratory of Disaster Monitoring and Mechanism Simulation, Baoji University of Arts and Sciences, Baoji 721013, China | | REN Xue-ying | Shaanxi Key Laboratory of Disaster Monitoring and Mechanism Simulation, Baoji University of Arts and Sciences, Baoji 721013, China | | WANG Zhou-feng | Key Laboratory of Subsurface Hydrology and Ecological Effect in Arid Region of Ministry of Education, Chang'an University, Xi'an 710054, China | | CAO Shu-miao | Key Laboratory of Subsurface Hydrology and Ecological Effect in Arid Region of Ministry of Education, Chang'an University, Xi'an 710054, China |
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| Abstract: |
| To study the relationship between plant antioxidant enzyme activity and combination heavy metal pollution, the effects of combination pollution by heavy metals Cd(0, 10, 20, 30, 40, 50 mg·kg-1), Zn(0, 150, 250, 350, 450, 550 mg·kg-1), and Pb(0, 100, 300, 500, 700, 900 mg·kg-1) on the activity of the enzymes catalase(CAT), peroxidase(POD), and superoxide dismutase(SOD) were investigated for different periods of stress(14, 21, 28, 35 d), based on orthogonal and interactive soil culture experiments. The results showed that CAT and POD were activated by Cd, whereas SOD was inhibited. CAT and POD were inhibited by Zn and Pb, whereas SOD was activated. Under the contamination stress of a combination of heavy metals, POD and SOD activities significantly increased with incubation time. In addition, at 28 and 35 d, POD and SOD activities were respectively 260% and 637.4% of those of the control. SOD activity significantly responded to the interactive effects of Cd×Zn, Cd×Pb, and Zn×Pb(P<0.05) and insignificantly responded to CAT and POD activities. The response of SOD activity was more sensitive than that of the other two antioxidant enzymes. Therefore, SOD activity can be used as an indicative index in the evaluation of the heavy metal contamination of soil. |
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