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| Effect of heavy metal-tolerant spore-forming bacteria on the cadmium and lead uptake of pepper |
| Received:October 30, 2017 |
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| KeyWord:Bacillus;pepper;cadmium;lead;immobilizing agent |
| Author Name | Affiliation | E-mail | | YANG Li | Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China | | | YAN Chuan-ming | Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China | | | HE Zhuo | Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China | | | SHENG Xia-fang | Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China | | | HE Lin-yan | Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China | helyan0794@njau.edu.cn |
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| Abstract: |
| In order to identify agents for reducing the accumulation of heavy metals in vegetables grown in contaminated soils, heavy metal-tolerant spore-forming bacteria were isolated from the rhizosphere of Capsicum annuum and Lycopersicon esculentum grown on farmland near mines in Nanjing, China, and the effects of the isolated bacteria on the growth and Cd and Pb uptake of pepper plants were assessed. Seven heavy metal-tolerant spore-forming bacterial strains were isolated and found to possess a variety of plant growth-promoting properties, such as phosphate solubilization and the production of indole acetic acid(IAA), siderophores, and polysaccharides. The bacteria were identified as Bacillus megaterium, B. aryabhattai, B. velezensis, and Lysinibacillus fusiformis, based on 16S rRNA gene sequences and phylogenetic analysis. The bacteria reduced the concentrations of water-soluble Cd and Pb in the solution by 30%~39% and 4.3%~25.6%, respectively, and strains L11, L15, and L44(B. megaterium) significantly promoted plant growth and reduced fruit concentrations of Cd and Pb. Furthermore, the lowest Cd(0.07 mg·kg-1) and Pb(0.2 mg·kg-1) levels were observed in the fruits of peppers inoculated with B. megaterium L44, which significantly reduced the heavy metals by 39.1% and 81.3%, respectively. Therefore, B. megaterium L44 is a more efficient agent for reducing the heavy metal levels of vegetables grown in contaminated soil. |
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