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| Immunotoxicity of di(2-ethylhexyl) phthalate to macrophages |
| Received:September 29, 2017 |
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| KeyWord:di(2-ethylhexyl)phthalate;macrophage;RAW264.7 cell;immunotoxicity |
| Author Name | Affiliation | E-mail | | HAN Jia-ying | High School Affliated to Huazhong Normal University, Wuhan 430223, China | | | SU Yi-ling | College of Life Science, Huazhong Normal University, Wuhan 430079, China | | | XIONG Li | College of Life Science, Huazhong Normal University, Wuhan 430079, China | | | GENG Hui | College of Life Science, Huazhong Normal University, Wuhan 430079, China | genghui@mail.ccnu.edu.cn |
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| Abstract: |
| To evaluate the potential immunotoxicity of di(2-ethylhexyl) phthalate(DEHP), RAW264.7 cells and peritoneal macrophages were treated with different concentrations of DEHP. Further, the phagocytosis efficiency and cytokine secretion and reactive oxygen species(ROS) production ability of RAW264.7 cells, and adherence ability and phagocytosis efficiency of peritoneal macrophages were determined. The results revealed that the phagocytosis efficiency of RAW264.7 cells treated with 1, 5 mg·L-1 and 10 mg·L-1 DEHP was significantly inhibited. The concentration of tumor necrosis factor(TNF)-α, interleukin(IL)-12, and IL-23 decreased significantly in the cell culture supernatant. Furthermore, there was a dose-effect relationship between DEHP exposure and concentration of TNF-α, IL12, and IL-23. The production of ROS in DEHP-treated RAW264.7 cells was inhibited significantly with increase in the concentration of DEHP. Further, DEHP inhibited the adhesion ability and phagocytosis efficiency of peritoneal macrophages collected form DEHP-treated mice. These results indicate that DEHP treatment can inhibit the normal function of both RAW264.7 cells and peritoneal macrophages. |
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