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| Screening of pyrene-degrading bacteria from Kochia scoparia rhizosphere in coking plant and study of plant growth-promoting ability |
| Received:July 17, 2017 |
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| KeyWord:plant growth-promoting rhizobacteria;Kochia scoparia;polycyclic aromatic hydrocarbons;degradation |
| Author Name | Affiliation | | SONG Li-chao | College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, China | | ZHANG Wei | College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, China | | NIU Xu-guang | College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, China | | LI Rui | College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, China | | CAO Yang | College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, China | | ZHAO Hao-yu | College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, China | | ZHOU Li-yang | College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, China |
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| Abstract: |
| In order to enhance in situ bioremediation of coking plant soils contaminated by polycyclic aromatic hydrocarbons(PAHs) and obtain plant growth-promoting bacteria that can degrade PAHs, functional strains in the rhizosphere of Kochia scoparia, which is a typical plant in coking plants, were obtained using the enrichment culture method. Pyrene and 1-amino cyclopropane-1-deaminase(ACC) were used as the sole carbon and nitrogen sources, respectively. The pyrene degradation ability and plant growth-promoting characteristics of the isolated strains were studied. The effect of the strains on the germination rate and root length of Kochia scoparia seeds were also analyzed using pyrene as the carbon source. After several enrichment times, 7 strains were identified and named as Kocuria sp. KSB1, Bacillus sp.KSB2, Myroides sp. KSB3, Serratia. sp. KSB4, Paracoccus sp. KSB5, Staphylococcus sciuri. KSB6, and Bacillus sp.KSB7. In the degradation experiment, more than 58% of pyrene could be degraded in 14 days by strains KSB1, KSB2, KSB4, KSB5, and KSB7. The activity of ACC deaminase was more than 3.5 M α-KB mg-1·h-1 in KSB2, KSB4, and KSB7. Strains KSB2, KSB4, and KSB7 significantly improved the germination rate and root length under pyrene stresses of 10~25 mg·L-1. Compared to CK, KSB7 increased the germination rate by 56.76% and shoot length by 88.9%, respectively. The results show that strain KSB7 has great potential in the bioremediation of contaminated soils of coking plants using Kochia scoparia. |
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