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| Concentration-ratio-dependent antagonism of sulfonamide antibiotics towards Vibrio qinghaiensis sp.-Q67 |
| Received:May 17, 2017 |
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| KeyWord:sulfonamide antibiotics;mixture;Vibrio qinghaiensis sp.-Q67;concentration-ratio dependency;antagonism |
| Author Name | Affiliation | E-mail | | DING Ting-ting | Key Laboratory of Water Pollution Control and Wastewater Resource of Anhui Province, College of Environment and Energy Engineering, Anhui Jianzhu University, Hefei 230601, China | | | ZHANG Jin | Key Laboratory of Water Pollution Control and Wastewater Resource of Anhui Province, College of Environment and Energy Engineering, Anhui Jianzhu University, Hefei 230601, China
Beijing Key Laboratory for Emerging Organic Contaminants Control, Tsinghua University, Beijing 100084, China | ginnzy@163.com | | DONG Xin-qi | Key Laboratory of Water Pollution Control and Wastewater Resource of Anhui Province, College of Environment and Energy Engineering, Anhui Jianzhu University, Hefei 230601, China | | | HONG Gui-yun | Key Laboratory of Water Pollution Control and Wastewater Resource of Anhui Province, College of Environment and Energy Engineering, Anhui Jianzhu University, Hefei 230601, China | | | BAO Li-ning | Key Laboratory of Water Pollution Control and Wastewater Resource of Anhui Province, College of Environment and Energy Engineering, Anhui Jianzhu University, Hefei 230601, China | |
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| Abstract: |
| To investigate the long-term toxicity of sulfonamide antibiotics(SAs) towards organisms, five SAs:sulfamethazine(SCP), sulfamethoxazole(SD), sulfasalazine(SPY), sulfadiazine(SMX), and sulfachloro pyridazine(SM2) were selected as research subjects. The direct equipartition ray method was used to design ten binary mixture systems, and five rays with different concentration ratios(pis) were arranged in each mixture system. The toxicities of the five SAs and their mixture systems towards the photobacterium Vibrio qinghaiensis sp.-Q67 were determined using the optimized long-term microplate toxicity analysis(L-MTA) method. Concentration addition was employed as the standard reference model to analyze the toxicity interaction within mixture systems. The results showed that the toxicities of the five SAs and their mixture rays displayed obvious differences after 16 h. Based on the negative logarithm of median effect concentration(pEC50), the toxicity order of the five SAs was as follows:SMX(pEC50=3.95) > SCP(pEC50=3.65) > SPY(pEC50=3.41) > SD(pEC50=3.36) > SM2(pEC50=3.21). Seven out of the ten mixture systems displayed additive action and the other three, SCP-SPY, SCP-SMX, and SCP-SM2, showed different antagonism. Antagonism of SCP-SPY and SCP-SMX gradually strengthened with the decrease of component SCP's pis from R1 to R2, and then was mostly stable from R2 to R5. The antagonism of the SCP-SM2 mixture system strengthened with the decrease of SCP's pis from R1 to R2, then declined gradually from R2 to R5, and synergism appeared in the relatively high concentration region of R5. |
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