|
| Characteristics of cellulose-degrading microbial communities in upland and paddy-upland rotation land soils in red soil hilly region |
| Received:April 05, 2017 |
| View Full Text View/Add Comment Download reader |
| KeyWord:cellulose;upland;upland-paddy rotation land;cellobiohydrolase;microbial community |
| Author Name | Affiliation | E-mail | | WANG Yu-qing | College of Biological Science and Technology, Hunan Agricultural University, Changsha 410128, China
The Key Laboratory of Subtropical Agro-Ecology, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha 410125, China | | | CHEN Xiang-bi | The Key Laboratory of Subtropical Agro-Ecology, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha 410125, China | xbchen@isa.ac.cn | | DONG Ming-zhe | The Key Laboratory of Subtropical Agro-Ecology, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha 410125, China | | | FENG Shu-zhen | The Key Laboratory of Subtropical Agro-Ecology, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha 410125, China
Guangxi University of Science and Technology, Liuzhou 545006, China | | | HU Ya-jun | The Key Laboratory of Subtropical Agro-Ecology, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha 410125, China | | | SU Yi-rong | The Key Laboratory of Subtropical Agro-Ecology, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha 410125, China | | | GE Ti-da | The Key Laboratory of Subtropical Agro-Ecology, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha 410125, China | | | ZHANG Zhen-hua | Southern Regional Collaborative Innovation Center for Grain and Oil Crops in China, Hunan Agricultural University, Changsha 410128, China | | | LI Qiao-yun | College of Biological Science and Technology, Hunan Agricultural University, Changsha 410128, China | 1065596897@qq.com |
|
| Hits: 2228 |
| Download times: 2504 |
| Abstract: |
| Cellulose is the most abundant organic component in crop residue and thus plays a vital role in organic matter transformation in a gricultural soils. The degradation of cellulose in soil is mainly driven by microorganisms. The fungal cbhI gene is one of the key microbial genes participating in cellulose degradation. Based on long-term field experiments in subtropical hilly region, two land use types(upland and paddy-upland rotation land) and two fertilization treatments(chemical fertilizers and chemical fertilizers combined with crop straw) were selected for this study. The annual dynamics of cellulose content, cellobiohydrolase activity, and abundance and composition of cellulose-degrading microbial communities containing cbhI in surface soils were analyzed to reveal the characteristics of microbial decomposition of cellulose in agricultural soils. Results showed that after 13 years of long-term fertilization, cellulose had not accumulated significantly. Annual dynamic data indicated the newly added cellulose was degraded or converted into other forms within 6 and 3 months after straw incorporation into upland and paddy-upland rotation land soils, respectively. Both the Pearson correlation analysis and stepwise linear regression analysis showed that cellulose contents were positively related to the activity of cellobiohydrolase and that abundances of the fungal cbhI gene were positively related to cellobiohydrolase activity(P<0.01). This indicated that the fungal cbhI gene may be a potentially corresponding indicator of microbial decomposition of cellulose in the tested agricultural soils. Cluster analysis showed that cellulose-degrading microbial communities in upland soils and paddy-upland rotation land soils were separated from each other, suggesting that land-use type is the key factor in shaping cellulose-degrading microbial communities as compared with the fertilization treatments. Cloning and sequencing data showed that Agaricomycetes and Sordariomycetes, which accounted for 22.9%~39.5%(average 34.7%) and 17.7%~42.3%(average 28.5%) of total clones, respectively, were the dominant cellulolytic microbial groups. Sordariomycetes may dominate the microbial process of cellulose decomposition after crop straw returns to the field. This study clarified the similarities and differences of cellulose decomposition and its functional microbial communities between upland and paddy-upland rotation land after straw returns to the field, and thus provided the basic data on revealing microbial mechanisms of labile soil organic matter(cellulose) turnover in agricultural soils. |
|
|
|
