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| Effects of transgenic maize containing cry1Ab and epsps gene C0030.3.5 on the abundance and diversity of soil archaea |
| Received:March 28, 2017 |
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| KeyWord:cry1Ab;epsps;transgenic maize;archaea;abundance;diversity |
| Author Name | Affiliation | E-mail | | WANG Jing | Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China
College of Agronomy & Resources and Environment, Tianjin Agricultural University, Tianjin 300384, China | | | WANG Rui | Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China
College of Agronomy & Resources and Environment, Tianjin Agricultural University, Tianjin 300384, China | | | ZHU Ke | Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China
College of Agronomy & Resources and Environment, Tianjin Agricultural University, Tianjin 300384, China | | | XIU Wei-ming | Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | | ZHAO Jian-ning | Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | | YANG Dian-lin | Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | | LI Gang | Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | ligang06@caas.cn | | TIAN Xiu-ping | College of Agronomy & Resources and Environment, Tianjin Agricultural University, Tianjin 300384, China | tian5418@sina.com |
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| Abstract: |
| In order to comprehensively understand the ecological safety of transgenic maize, soil samples were collected in 2015 at the jointing, tassel, milky and ripening stage of transgenic maize containing cry1Ab and epsps gene C0030.3.5, and the effects of this maize on the abundance and diversity of soil archaea were investigated by quantitative polymerase chain reaction and terminal restriction fragment length polymorphism(T-RFLP) analyses. The results indicated that the number of archaea in the rhizosphere and non-rhizosphere zones of transgenic maize containing cry1Ab and epsps gene C0030.3.5(TM) and the parental maize DBN318(PM) was between 1.41×109 and 4.04×109 copies·g-1 soil, and all showed the trend of initially increasing and then decreasing with growth stage progression. No significant difference was observed in the abundance of the archaeal 16S rRNA gene between TM and PM for the same growth stage and sampling area(P>0.05). A total of 15 terminal restriction fragments of different lengths were acquired by T-RFLP, and among these, the fragments with lengths of 89 bp and 184 bp were derived from the dominant populations. For the same growth stage and sampling area, each dominant population showed no significant difference between TM and PM(P>0.05). The Shannon index initially decreased, then increased and decreased again, with the exception of the non-rhizosphere zone of PM, which showed an initial decrease followed by an increase. The Evenness index of the rhizosphere of TM and PM initially decreased and then increased. However, in the non-rhizosphere zone of TM and PM, the Evenness index showed a decrease-increase-decrease trend. For both the Shannon and Evenness indices, there were no significant differences between TM and PM for the same growth stage and sampling area. Redundancy analysis indicated significant correlations between total nitrogen and nitrate-nitrogen and the composition of archaea. Principal component analysis indicated no significant separation between TM and PM in either the rhizosphere or non-rhizosphere zone, suggesting that there was no significant difference in the composition of archaea between TM and PM. In summary, no significant differences were found in the abundance and community structure of archaea between the transgenic maize containing the cry1Ab and epsps genes and its parental maize. The abundance and diversity indices of archaea were mainly affected by the growth stage of maize. Soil total nitrogen and nitrate-nitrogen were the key factors controlling the soil archaeal community. |
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