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| Effect of Pb and Cd on DNA methylation of Isoetes sinensis, a rare plant |
| Received:September 05, 2016 |
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| KeyWord:Isoetes sinensis Palm;DNA methylation;MSAP;Pb;Cd |
| Author Name | Affiliation | E-mail | | DING Guo-hua | Key Laboratory of Plant Biology in Colleges of Heilongjiang Province, Harbin 150025, China
School of Life Science & Technology, Harbin Normal University, Harbin 150025, China | | | GUO Dan-di | Key Laboratory of Plant Biology in Colleges of Heilongjiang Province, Harbin 150025, China | | | GUAN Yang | Key Laboratory of Plant Biology in Colleges of Heilongjiang Province, Harbin 150025, China
School of Life Science & Technology, Harbin Normal University, Harbin 150025, China | | | LIU Bao-dong | Key Laboratory of Plant Biology in Colleges of Heilongjiang Province, Harbin 150025, China
School of Life Science & Technology, Harbin Normal University, Harbin 150025, China | | | CHI Chun-yu | Key Laboratory of Plant Biology in Colleges of Heilongjiang Province, Harbin 150025, China
School of Life Science & Technology, Harbin Normal University, Harbin 150025, China | chichunyu2006@126.com |
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| Abstract: |
| To investigate the molecular response of ancient plants to heavy metal stress and to explore the feature of DNA methylation in endangered plants exposure to heavy metals, Isoetes sinensis, an endangered plant, was stressed with three different concentrations of two heavy metals Pb and Cd, respectively. Then the degree of DNA methylation in the leaves were measured on the 28th day using Methylation Sensitive Amplified Polymorphism(MSAP) technique. The results showed that the DNA methylated profile of I. sinensis was affected by heavy metal stress. There was no significant difference in the amount of DNA methylation between the test and control groups(CK, Pb and Cd was 46.96%, 48.23% and 48.1%, respectively), however, full-methylation level(Pb 28.34%, Cd 20.25%) were lower than control(33.91%), in contrast, hemi-methylation level(Pb 19.89%, Cd 27.85%) were higher than control(13.04%). The change of patterns from no methylation or hemi-methylation of inner and outer cytosines into full-methylation of inner and outer cytosines accounted for a large proportion in enhanced methylation aspects and that from full-methylation of inner and outer cytosines into no methylation or hemi- or full-methylation of inner cytosines occupied most of demethylation. The proportion of DNA methylation increased by both Pb and Cd stresses is nearly equal(39.04% and 39.71%), but the proportion of DNA demethylation by Cd is higher than that by Pb(46.86% than 33.92%). |
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