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| Development of a enzyme-linked immunosorbent assay for diethyl phthalate analysis in water and sediment samples from rivers in Zhenjiang City |
| Received:March 10, 2016 |
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| KeyWord:plasticizer, DEP, antibody |
| Author Name | Affiliation | E-mail | | ZENG Kun | College of Environmental and Safety Engineering, Jiangsu University, Zhenjiang 212013, China
Institute of Environment and Ecology, Jiangsu University, Zhenjiang 212013, China | | | ZHOU Jun | College of Environmental and Safety Engineering, Jiangsu University, Zhenjiang 212013, China | | | SHAO Jie | College of Environmental and Safety Engineering, Jiangsu University, Zhenjiang 212013, China | | | ZHANG Zhen | College of Environmental and Safety Engineering, Jiangsu University, Zhenjiang 212013, China | zhzhenok@163.com |
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| Abstract: |
| Based on a polyclonal antibody targeting diethyl phthalate(DEP), a sensitive indirect competitive enzyme-linked immunosorbent assay(ELISA) was proposed and several parameters were investigated. Under the optimized conditions, the developed method showed high sensitivity to DEP(the limit of detection, 8.2 ng·mL-1) and high selectivity with cross-reactivities less than 4.29% to seven structurally related phthalate esters. The linear range was from 20 ng·mL-1 to 320 ng·mL-1. The recoveries(90.46%~122.75%) and reproducibility(CV of intra-assay,3.38%~9.09%; CV of inter-assay, 3.76%~12.78%) were satisfactory. Our proposed ELISA indicated that it had good agreement with those given by GC-MS, which was applied to detect DEP in waters and sediments from ancient canal and Yunliang River. And DEP was found in most of target areas(the detection rate, 62.5% in waters and 87.5% in sediments), the concentration was up to 6.61 ng·mL-1 and 86.9 ng·g-1 in water and sediments, respectively. |
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