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| Chemotaxis of Arthrobacter sp. CN2 towards p-nitrophenol |
| Received:April 17, 2016 |
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| KeyWord:Arthrobacter sp.;p-nitrophenol;chemotaxis;environmental bioremediation |
| Author Name | Affiliation | E-mail | | QIAO Cheng | Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China | | | REN Lei | Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China | | | JIA Yang | Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China | | | FAN Shuang-hu | Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China | | | WANG Jun-huan | Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China | | | YAN Yan-chun | Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China | yanyanchun@caas.cn |
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| Abstract: |
| Microbial chemotactic systems are critical for bacteria to sense chemicals in the surrounding medium, which enable them to move towards or away from certain chemotant. Strain Arthrobacter sp. CN2, which could efficiently degrade p-nitrophenol,was used in swarm plate assay, soil chemotaxis assay and capillary chemotaxis assay with p-nitrophenol as the sole substrate, respectively. In the meanwhile, a test of in situ soil assay was carried out in order to assess the application performance of CN2. High Performance Liquid Chromatography(HPLC) test revealed the ability of strain CN2 to degrade over 99% of p-nitrophenol within 72 h. A series of chemotaxis assays indicated that strain CN2 had obvious chemotaxis toward p-nitrophenol. Specifically in the capillary assay, the strain was positively chemotactic to p-nitrophenol within a certain range of concentrations(5~800 mg·L-1), but was gradually inhibited when the concentration of p-nitrophenol was higher than 800 mg·L-1. Furthermore, test of in situ soil assay showed a higher degradation efficiency in unsterilized soil than in sterilized soil where the degradation rate could reach up to 95% within 14 days. Our data revealed an excellent adaptability of CN2 to the environment and claimed the potential application in environmental remediation of pollutants. |
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