|
| Effects of antibiotics on the estradiol degradation by strain JX-2 |
| Received:May 05, 2016 |
| View Full Text View/Add Comment Download reader |
| KeyWord:antibiotics;estradiol-degrading bacterium;degradation;minimum inhibitory concentration;sewage |
| Author Name | Affiliation | E-mail | | LIU Jing-xian | Institute of Organic Contaminant Control and Soil Remediation, Nanjing Agricultural University, Nanjing 210095, China | | | LIU Juan | Institute of Organic Contaminant Control and Soil Remediation, Nanjing Agricultural University, Nanjing 210095, China | | | SUN Min-xia | Institute of Organic Contaminant Control and Soil Remediation, Nanjing Agricultural University, Nanjing 210095, China | | | GAO Yan-zheng | Institute of Organic Contaminant Control and Soil Remediation, Nanjing Agricultural University, Nanjing 210095, China | | | LI Shun-yao | Institute of Organic Contaminant Control and Soil Remediation, Nanjing Agricultural University, Nanjing 210095, China | | | LING Wan-ting | Institute of Organic Contaminant Control and Soil Remediation, Nanjing Agricultural University, Nanjing 210095, China | lingwanting@njau.edu.cn |
|
| Hits: 2403 |
| Download times: 2336 |
| Abstract: |
| The aim of this work was to investigate the effects of antibiotics on the estradiol(E2) degradation by an estradiol-degrading bacterium Rhodococcus sp. JX-2. The minimum inhibitory concentration(MIC) of 12 kinds of antibiotics to strain JX-2 was measured by the drug sensitive test. The influence of antibiotics at different concentrations on cell growth and E2-degradation performance by JX-2 was studied using an E2-antibiotics co-cultivation method. The impacts of mixed antibiotics at different concentrations and processing time on E2 degradation in both aqueous solution and sewage were elucidated. Strain JX-2 was observed to be extremely sensitive to penicillin and erythromycin. The drug resistance of strain JX-2 to chloramphenicol, oxytetracycline and sulfadiazine versus other antibiotics was relatively stronger. No significant influence was observed on the growth and E2-degradation performance of strain JX-2 under the stress of antibiotics at low concentrations, but high antibiotic concentrations obviously inhibited the strain JX-2 growth and reduced its E2-degradation performance. Extending the incubation time weakened the inhibitory effects of antibiotics at low concentrations on E2 degradation, while no such effects were observed for antibiotics at the high concentrations. The mixed antibiotics did not significantly influence the E2 degradation by strain JX-2 in aqueous solution when the antibiotics concentrations were 10.0 μg·L-1, 100.0 μg·L-1 and 1.0 mg·L-1, respectively. However, the mixed antibiotics significantly inhibited the E2 degradation when the antibiotics concentration increased to 10.0 mg·L-1, and the E2 degradation rate was only 48.2% in 7 days. Strain JX-2 performed well on E2 degradation in sewage when the antibiotics concentrations were 10.0 μg·L-1, 100.0 μg·L-1 and 1.0 mg·L-1, and the degradation rates were higher than 80%. |
|
|
|
