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Effects of biochar on chlorophyll and protective enzyme activity of rape seedlings in red soil under copper stress
Received:September 29, 2015  
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KeyWord:copper stress;rape;biochar;chlorophyll;antioxidant enzyme activity;malondialdehyde
Author NameAffiliationE-mail
WANG Xiao-wei Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang 330045, China
College of Agriculture, Jiangxi Agricultural University, Nanchang 330045, China 
 
XU Jian-cheng Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang 330045, China
College of Agriculture, Jiangxi Agricultural University, Nanchang 330045, China 
 
SUN Dan-ping Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang 330045, China
College of Agriculture, Jiangxi Agricultural University, Nanchang 330045, China 
 
LUO Yu-hang College of Agriculture, Jiangxi Agricultural University, Nanchang 330045, China  
LONG Chang-zhi College of Agriculture, Jiangxi Agricultural University, Nanchang 330045, China  
LU Mei-juan College of Territorial Resources and Environmental, Jiangxi Agricultural University, Nanchang 330045, China  
YANG Wen-ting Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang 330045, China
College of Agriculture, Jiangxi Agricultural University, Nanchang 330045, China 
ywt111@163.com 
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Abstract:
      A pot experiment was carried out to study the effects of biochar additions on chlorophyll content, antioxidant enzyme activity[superoxide dismutase(SOD), catalase(CAT), and peroxidase(POD)], soluble protein content and malondialdehyde(MDA) content in rape(Brassica campestris L.) seedlings in red soil under copper stress(300 mg·kg-1). The biochar was applied at 0, 2%, and 5%(m/m). Copper stress decreased content of chlorophyll a and b and total chlorophyll, but increased SOD, CAT, POD, MDA, and soluble protein content in rape leaves. Compared with no biochar addition, applying biochar increased the content of chlorophyll a and b and total chlorophyll, but reduced the activities of SOD, CAT, POD, and MDA, and the content of soluble protein in rape leaves. In conclusion, biochar applications improve chlorophyll content of rape seedlings, alleviate copper stress on rape seedlings, and maintain rape seedling normal growth under 300 mg Cu·kg-1 stress.