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| Effects of ventilation on nitrogen transformation and nirK gene diversity and abundance during composting process |
| Received:October 18, 2015 |
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| KeyWord:ventilation;composting;nirK;nitrogen transformation |
| Author Name | Affiliation | E-mail | | WANG Guo-xing | College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163000, China | | | DONG Gui-jun | Plant Protection and Inspection Station, Heilongjiang Land Reclamation Bureau, Harbin 150000, China | | | AI Shi-qi | College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163000, China | | | XIONG Zhi-qiang | College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163000, China | | | YAN Lei | College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163000, China | | | GAO Ya-mei | College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163000, China | | | WANG Yan-jie | College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163000, China | | | WANG Wei-dong | College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163000, China | wwdcyy@126.com |
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| Abstract: |
| Ventilation is one of the most important parameters affecting composting process. In this study, the effects of ventilation on nitrogen transformation and loss and nirK gene diversity and abundance during composting process were examined. Three treatments, CK:0 L·min-1·kg-1(Static compost), TF1:0.05 L·min-1·kg-1(Microaerobic compost) and TF4:0.2 L·min-1·kg-1(Aerobic compost), were designed. The content of ammonium-nitrogen, nitrate nitrogen, and total nitrogen during different periods were measured. The abundance of nirK gene during different periods and the diversity of nirK gene on the 63rd day of composting were analyzed. Results showed that the NH3 emissions and total nitrogen losses in three treatments increased with increasing amount of ventilation. The highest content of NO3--N and TN was observed in CK, TF1, and TF4 on the 63rd day, and was significantly affected by ventilation.The OTU number of nirK gene clone library of the 63rd day samples from CK, TF1 and TF4 was 9, 5, and 3, respectively. During the thermophilic and maturity periods, the abundance of nirK gene was negatively correlated with ventilation amount. The present results showed that ventilation significantly affects the transformation of nitrogen, and the diversity and abundance of nirK gene during composting, and increases TN and NO3--N content. |
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