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| Diversity of bacterial community in rice paddy soil grown with P-efficient transgenic rice(OsPT4) |
| Received:October 14, 2015 |
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| KeyWord:P-efficient transgenic rice;soil bacteria;PCR-DGGE;bacterial diversity |
| Author Name | Affiliation | E-mail | | ZANG Huai-min | Key Laboratory of Original Agro-environment Quality, Ministry of Agriculture/Tianjin Key Laboratory of Agro-environment and Agro-product Safety, Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | | LI Gang | Key Laboratory of Original Agro-environment Quality, Ministry of Agriculture/Tianjin Key Laboratory of Agro-environment and Agro-product Safety, Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | | XIU Wei-ming | Key Laboratory of Original Agro-environment Quality, Ministry of Agriculture/Tianjin Key Laboratory of Agro-environment and Agro-product Safety, Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | | WEI Lin-lin | Key Laboratory of Original Agro-environment Quality, Ministry of Agriculture/Tianjin Key Laboratory of Agro-environment and Agro-product Safety, Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | | NI Tu | Key Laboratory of Original Agro-environment Quality, Ministry of Agriculture/Tianjin Key Laboratory of Agro-environment and Agro-product Safety, Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | | YANG Dian-lin | Key Laboratory of Original Agro-environment Quality, Ministry of Agriculture/Tianjin Key Laboratory of Agro-environment and Agro-product Safety, Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | | ZHAO Jian-ning | Key Laboratory of Original Agro-environment Quality, Ministry of Agriculture/Tianjin Key Laboratory of Agro-environment and Agro-product Safety, Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | zhaojn2008@163.com |
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| Abstract: |
| The effects of genetically modified crops(GMCs) on soil microbial community have been received more and more attentions in recent years. In this study, the effects of P-efficient transgenic rice(OsPT4) on richness and evenness of soil bacterial community were compared with parental OsPT4(Nipponbare) and P-efficient mutant rice(PHO2), using denaturing gradient gel electrophoresis(DGGE) technique. Two phosphorous(P) application rates(0 and 15 g·m-2) were used. The bacterial 16S rDNA DGGE fingerprintings had no significant differences in community structure between P-efficient transgenic rice, Nipponbare and P-efficientmutant rice at the same P rate during the same growing stage. Under P application, PHO2 had one more electrophoretic band than OsPT4 and Nipponbare did at the elongation stage. This increased band belonged to Cyanobacterium of Cyanobactteria. At the heading and flowering stages, OsPT4 had one less electrophoretic band than Nipponbare did. This missed band was a part of Gemmatimonadetes. Under no P application, OsPT4 emerged four electrophoretic bands during both tillering and maturing stages, as compared with Nipponbare. These four electrophoretic bands were associated with Geobacter of Proteobacteria, Bacillus of Firmicutes, Chloroflexi, and Geobacter of Proteobacteria. This study demonstrates that P-efficient transgenic rice(OsPT4) has significant effects on richness(S), Shannon-wiener index(H) and evenness(EH) of soil bacteria at certain rice growth stages, and that P fertilizer may also be involved in these effects. |
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