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| Effects of cadmium stresses on physiological characteristics and genomic DNA polymorphism of Brassica chinensis L. seedlings |
| Received:October 13, 2015 |
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| KeyWord:Brassica chinensis;Cd stress;physiological characteristics;RAPD ;DNA polymorphism |
| Author Name | Affiliation | | ZHANG Yun-fang | School of Life Sciences, Huaibei Normal University, Anhui Key Laboratory of Resources and Plant Biology, Huaibei 235000, China | | CHEN Chu | School of Life Sciences, Huaibei Normal University, Anhui Key Laboratory of Resources and Plant Biology, Huaibei 235000, China | | CHEN Chen | School of Life Sciences, Huaibei Normal University, Anhui Key Laboratory of Resources and Plant Biology, Huaibei 235000, China | | SHU Liang-zuo | School of Life Sciences, Huaibei Normal University, Anhui Key Laboratory of Resources and Plant Biology, Huaibei 235000, China |
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| Abstract: |
| Cadmium(Cd) is a toxic trace pollutant that can easily be taken up by vegetable crops. The effects of Cd stresses on some physiological characteristics and genomic DNA polymorphism in Brassica chinensis cultivar "Suzhouqing" seedlings were studied by random amplified polymorphic DNA(RAPD) technique and physiological tests. Results showed that treating plants with 1, 5, 10, 20 and 40 mg·L-1 Cd for 20 days inhibited fresh weight and root elongation of seedlings, decreased chlorophyll content and total soluble protein content, but increased malondialdehyde content and electrolyte leakage. Using 8 oligonucleotide primers(10 bp) to amplify the genomic DNA of the seedlings by polymerase chain reaction, 55 clear RAPD bands were detected in normal seedlings. Compared with the control, the RAPD profiles of Cd-exposed seedlings showed new bands, lost normal bands, altered band intensities. In addition, DNA polymorphism was dose-dependent. The genomic DNA template stability decreased with increasing Cd concentrations, and the genomic DNA polymorphism of Brassica chinensis was positively correlated with some physiological characteristics under Cd stresses. In conclusion, the DNA polymorphisms detected by RAPD technique could be used as a biomarker for detecting the genotoxic effects of Cd on Brassica chinensis. |
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