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| Degradation of Rice Straw by Degrading Strains Isolated by a “Hanging-Enrichment” Method |
| Received:April 13, 2015 |
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| KeyWord:degrading microorganisms;rice straw;cellulase;hemicellulase;degradation |
| Author Name | Affiliation | E-mail | | WEI Zhong | National Engineering Research Center for Organic-based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Utilization, Nanjing Agricultural University, Nanjing 210095, China | | | XU Chun-miao | National Engineering Research Center for Organic-based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Utilization, Nanjing Agricultural University, Nanjing 210095, China | | | ZHENG Hai-ping | National Engineering Research Center for Organic-based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Utilization, Nanjing Agricultural University, Nanjing 210095, China | | | LIAO Han-peng | National Engineering Research Center for Organic-based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Utilization, Nanjing Agricultural University, Nanjing 210095, China | | | WANG Shi-mei | National Engineering Research Center for Organic-based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Utilization, Nanjing Agricultural University, Nanjing 210095, China | | | SHEN Qi-rong | National Engineering Research Center for Organic-based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Utilization, Nanjing Agricultural University, Nanjing 210095, China | | | XU Yang-chun | National Engineering Research Center for Organic-based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Utilization, Nanjing Agricultural University, Nanjing 210095, China | ycxu@njau.edu.cn |
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| Abstract: |
| In this study, a "Hanging-enrichment" method was developed to isolate and screen high-performance straw-degrading strains. By utilizing rice straw powder as sole carbon source, twelve strains with abilities to degrade straw were obtained. Then two bacterial (GS2-3 and ZJA-6) and two fungal(JB-5 and ZJC-1) strains with better degradation performance were selected for further studies. The strains GS2-3, ZJA-6, JB-5 and ZJC-1 were preliminarily identified as Bacillus amyloliquefaciens, Paenibacillus pabuli, Trichoderma harzianum, and T. koningiopsis, respectively, based on the morphological observation and 16S rDNA and ITS sequencing. Cellulase activity was 21.85, 13.20, 106.48, and 187.13 U·mL-1, and hemicellulase activity was 960.70, 1 879.67, 100.64, and 6 727.30 U· mL-1, for the strains GS2-3, ZJA-6, JB-5, and ZJC-1, respectively. The relative degradation rates of rice straw by strains GS2-3, ZJA-6, ZJB-5, and ZJC-1 were 22.78%, 34.25%, 33.32%, and 27.99%, respectively, all significantly higher than those of other strains after 7 day incubation in liquid medium. The rice straw degradation experiment in soil showed that these strains had stronger ability to degrade rice straw. Under soil conditions, the degradation rate was 20%, 18%, 15.2%, and 24% for the strains GS2-3, ZJA-6, ZJB-5, and ZJC-1 after 28 d, respectively. In conclusion, "Hanging-enrichment" method could be a potential approach to obtaining rice straw degrading microorganisms. |
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