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| Enhancement of Enzymatic Hydrolysis of Corn Stover by Liquid Hot Water Pretreatment and Ethanol Extraction |
| Received:March 13, 2015 |
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| KeyWord:biomass;hydrothermal;pretreatment;ethanol extraction;corn stover;enzymatic hydrolysis |
| Author Name | Affiliation | E-mail | | LI Zi-mu | State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China | | | YU Yan-ling | State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China
School of Chemical Engineering & Technology, Harbin Institute of Technology, Harbin 150001, China | yyl0206@163.com | | ZHAO Gui-hong | State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China
College of environmental and chemical engineering, Heilongjiang University of Science and Technology
Harbin 150022, China | | | SUN Jia-xing | School of Chemical Engineering & Technology, Harbin Institute of Technology, Harbin 150001, China | | | LI Dong-mei | School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China | | | HUANG Yu-dong | School of Chemical Engineering & Technology, Harbin Institute of Technology, Harbin 150001, China | | | FENG Yu-jie | State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China | yujief@hit.edu.cn |
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| Abstract: |
| Lignin could not be significantly removed from lignocellulose by liquid hot water(LHW) pretreatment, but lignin would migrate from cell wall and redeposit on the surface of cell wall at high temperature during pretreatment. To take advantage of this characteristic, we examined the changes of main components and enzymatic hydrolysis of corn stover pretreated by LHW pretreatment before and after ethanol extraction.Ethanol extraction process was also optimized. Compared to LHW pretreatment, ethanol extraction removed some lignins from, increased cellulose content in and improved cellulose digestibility of corn stover. Lignin removal increased with increasing temperature of LHW pretreatment. The optimal ethanol extraction was as follows:25 of liquid-solid ratio, room temperature, 120 r·min-1, and 10 h. For corn stover pretreated at 210 ℃ for 20 min, ethanol extraction decreased lignin from 30.5% to 18.2%, while increased cellulose content from 62.2% to 73.6%. After ethanol extraction, the enzymatic hydrolysis time was shortened from 48h to 24h. The cellulose digestibility reached 93.2%(15 FPU·g-1 cellulose). |
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