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| Cell Fusion of PAHs-degrading Bacteria and Its Degradation Ability |
| Received:January 22, 2015 |
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| KeyWord:phenanthrene;pyrene;fusant strain;genetic marker;PAHs |
| Author Name | Affiliation | E-mail | | LU Jing | School of Chemical Engineering and Environment, North University of China, Taiyuan 030051, China | | | HOU Bin | School of Chemical Engineering and Environment, North University of China, Taiyuan 030051, China | houbin566@163.com | | GUO Chu-ling | School of Environment and Energy, South China University of Technology, Guangzhou 510006, China | |
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| Abstract: |
| In the present study, a phenethrene-degradation strain(Sphingomonas sp.) GY2B and a pyrene-degradation strain(Pseudomonas sp.) GP3A were used to obtain a fusant capable of degrading PAHs. The antibiotic resistance test was used to screen the genetic markers. Morphology and molecular biology were employed to identify fusants. Results showed that the genetic markers of Sphingomonas sp. GY2B was piperacillin(80 μg·mL-1)-resistant and Pseudomonas sp. GP3A was ceftazidime(80 μg·mL-1)- or erythromycin(100~150 μg·mL-1)-resistant, respectively. A fusant strain with the highest degradation of both phenanthrene and pyrene was selected and named F14. Tests by colony morphology, electron microscope, scanning electron microscope(SEM) and molecular technology(PCR-RFLP) indicated that the strain F14 was a fusant of GP3A and GY2B, and was different from its parents. F14 could degrade both phenanthrene and pyrene. It almost completely degraded phenanthrene(100 μg·mL-1) within 24 hours, much quicker than GY2B and other strains. F14 degraded 18% of pyrene(100 mg·L-1) within 10 days, still higher than GP3A. |
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