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| Metal Accumulation and Expressions of Root Two Key Transporter Genes, HMA4 and IRT1, of High/Low Cd-Accumulating Amaranth Cultivars |
| Received:December 06, 2014 |
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| KeyWord:edible amaranth;Cd accumulation;IRT1;HMA4;cultivar difference |
| Author Name | Affiliation | E-mail | | CHEN Yan-fang | School of Environment, Jinan University, Guangzhou 510632, China
Key Laboratory of Water/Soil Toxic Pollutants Control and Bioremediation, Department of Education of Guangdong Province, Guangzhou 510632, China | | | LI Qu-sheng | School of Environment, Jinan University, Guangzhou 510632, China
Key Laboratory of Water/Soil Toxic Pollutants Control and Bioremediation, Department of Education of Guangdong Province, Guangzhou 510632, China | liqusheng@21cn.com | | HE Bao-yan | School of Environment, Jinan University, Guangzhou 510632, China
Key Laboratory of Water/Soil Toxic Pollutants Control and Bioremediation, Department of Education of Guangdong Province, Guangzhou 510632, China | | | MEI Xiu-qin | School of Environment, Jinan University, Guangzhou 510632, China
Key Laboratory of Water/Soil Toxic Pollutants Control and Bioremediation, Department of Education of Guangdong Province, Guangzhou 510632, China | | | LEI Yong-kang | School of Environment, Jinan University, Guangzhou 510632, China
Key Laboratory of Water/Soil Toxic Pollutants Control and Bioremediation, Department of Education of Guangdong Province, Guangzhou 510632, China | | | XU Zhi-min | School of Environment, Jinan University, Guangzhou 510632, China
Key Laboratory of Water/Soil Toxic Pollutants Control and Bioremediation, Department of Education of Guangdong Province, Guangzhou 510632, China | | | ZHOU Li-zhen | School of Environment, Jinan University, Guangzhou 510632, China
Key Laboratory of Water/Soil Toxic Pollutants Control and Bioremediation, Department of Education of Guangdong Province, Guangzhou 510632, China | |
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| Abstract: |
| Metal accumulation in plants may be controlled by their transporter gene expressions. Here a pot experiment was conducted to investigate the differences of metal uptake by edible amaranth cultivars in relation to the expressions of two key transporter genes, HMA4 and IRT1, in roots using garden soil polluted with heavy metals. The expressions of genes IRT1 and HMA4 in root were determined by semi-quantitative PCR method. Cultivars Baiguxiaoyuanye and Zhengtaixiaoyuanye accumulated (0.116±0.007)mg·kg-1 and (0.146±0.002)mg·kg-1(fresh weight) Cd in shoot, but Taiwanbai and Hongliuye contained (0.210±0.005)mg·kg-1 and (0.349±0.026)mg·kg-1 (fresh weight)Cd in shoot, respectively. Therefore, the later two are high-Cd accumulating cultivars. Cadmium accumulation in root protoplasts was positively correlated with Fe and Pb(P<0.01). Concentrations of Cd, Fe and Pb in and the expressions of gene IRT1 in root protoplasts of high-Cd accumulating cultivar Hongliuye were significantly higher than those in low-Cd accumulating cultivar Baiguxiaoyuanye. This implied that higher expression of gene IRT1 in root might contribute to Fe uptake by root, as well as Cd and Pb uptake. Cadmium accumulation in shoot was significantly positively correlated with Zn and Fe(P<0.05) and extremely significantly with Pb, Ca and Mn(P<0.01). Concentrations of Cd, Ca, Fe, Mn and Pb in shoot of high-Cd accumulating cultivar Hongliuye and its expression of gene HMA4 were significantly higher than those in low-Cd accumulating cultivar Baiguxiaoyuanye. This suggests that the higher expression of gene HMA4 in root may facilitate Cd and other metals transport from roots to shoots. |
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