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| Responses of Glutathione System of Aspergillus niger TL-F2 to Cu(Ⅱ) Stress |
| Received:December 12, 2014 |
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| KeyWord:Aspergillus niger TL-F2;anti-oxidation;glutathione system;copper |
| Author Name | Affiliation | E-mail | | LI Ding-xin | College of Resources and Environment, Anhui Agricultural University, Hefei 230036, China | | | FAN ting | College of Resources and Environment, Anhui Agricultural University, Hefei 230036, China | fanting@ahau.edu.cn | | TANG Zi-yang | College of Resources and Environment, Anhui Agricultural University, Hefei 230036, China | | | WANG Zhen | College of Resources and Environment, Anhui Agricultural University, Hefei 230036, China | | | CHEN Hai-yan | College of Resources and Environment, Anhui Agricultural University, Hefei 230036, China | | | LU Hong-juan | College of Resources and Environment, Anhui Agricultural University, Hefei 230036, China | | | ZHANG Guo-yi | College of Resources and Environment, Anhui Agricultural University, Hefei 230036, China | | | YE Wen-ling | College of Resources and Environment, Anhui Agricultural University, Hefei 230036, China | | | ZHANG Wei | College of Resources and Environment, Anhui Agricultural University, Hefei 230036, China | |
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| Abstract: |
| Aspergillus niger is widely used in removal of heavy metals from wastewater and polluted soil. A strain resistant to copper ions of high concentrations, named TL-F2, was isolated from a copper tailing. The effects of initial Cu(Ⅱ) concentrations(50, 100, 150, 200, 250, 300 mg·L-1) on growth and antioxidant system of Aspergillus niger TL-F2(A. niger TL-F2) were investigated at different growth stages. With increasing Cu(Ⅱ) concentrations, both biomass and soluble protein content of A. niger TL-F2 decreased, but malondialdehyde(MDA)content in A. niger TL-F2 increased. Compared with the control, the activity of GSH increased at all time. However, the content of oxidized glutathione(GSSG) and the activities of GPx and glutathione reductase(GR) displayed “initial increase and then decrease” over Cu(Ⅱ)concentrations and culture time. The concentration of GSSG was the highest of 365.68 μmol·L-1 at Cu(Ⅱ) concentration of 150 mg·L-1, while the activities of GPx and GR reached their maximum at Cu(Ⅱ) concentration of 100 mg·L-1. The ratio of GSH/GSSG decreased with increasing Cu(Ⅱ) concentrations and culture time. In summary, the GSH/GPx system in A.niger TL-F2 produced oxidative stresses to resist the excessive reactive oxygen species(ROS) via regulating the content or activities of GSH, GSSG, GPx and GR, under Cu(Ⅱ) stresses. It suggests that GSH/GPx system plays an important role in the antioxidant system in A. niger TL-F2 exposed to Cu(Ⅱ), and the ratio of GSH/GSSG values can also be an indicator of Cu(Ⅱ) toxicity to A. niger TL-F2. |
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