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| Immobilization of Fluoranthene-Degrading Herbaspirillum chlorophenolicum strain FA1 and Its Optimization |
| Received:November 23, 2014 |
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| KeyWord:fluoranthene;Herbaspirillum chlorophenolicum strain FA1;biodegradation;immobilization;response surface methodology |
| Author Name | Affiliation | | XU Hong-xia | Key Laboratory of Surficial Geochemistry, Ministry of Education
School of Earth Sciences and Engineering, Nanjing University, Nanjing 210023, China | | LI Xiao-hui | Key Laboratory of Surficial Geochemistry, Ministry of Education
School of Earth Sciences and Engineering, Nanjing University, Nanjing 210023, China | | SUN Yuan-yuan | Key Laboratory of Surficial Geochemistry, Ministry of Education
School of Earth Sciences and Engineering, Nanjing University, Nanjing 210023, China | | SHI Xiao-qing | Key Laboratory of Surficial Geochemistry, Ministry of Education
School of Earth Sciences and Engineering, Nanjing University, Nanjing 210023, China | | WU Ji-chun | Key Laboratory of Surficial Geochemistry, Ministry of Education
School of Earth Sciences and Engineering, Nanjing University, Nanjing 210023, China |
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| Abstract: |
| Microbial remediation of polycyclic aromatic hydrocarbons(PAHs), a group of persistent toxic contaminants that are widespread in terrestrial and aquatic environments, has received increasing interests. A fluoranthene-degrading strain FA1 previously isolated from activated sludge has showed capability to use fluoranthene as sole carbon and energy sources and to degrade fluoranthene in the presence of indigenous flora. In this study, immobilization of fluoranthene-degrading strain FA1 by various matrices and its effects on fluoranthene degradation in soil-water system were investigated. Results showed that the strain FA1 immobilized on polyvinyl alcohol(PVA)-diatomite matrix prepared by chemical method(boric acid) exhibited the highest degradation of fluoranthene, with a 97.74% removal of fluoranthene from soil-water system after 25 d. PVA-diatomite and PVA-activated carbon matrices obtained by chemical method showed better effectiveness than those obtained by freezing and thawing method. The optimal immobilization conditions of PVA-diatomite matrix(chemical method) were 9.6% of cell density, 11.2% of PVA, 4.5% of diatomite and 4.0 mm of bead size. Under these conditions, 99.46% of fluoranthene was removed from the soil-water system after 20 d. |
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