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| Effects of Three Heavy Metals on mRNA Expressions of Two Metallothioneins in Oxya chinensis |
| Received:October 13, 2014 |
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| KeyWord:Oxya chinensis;heavy metals;metallothionein;testis;ovary;muscle |
| Author Name | Affiliation | E-mail | | LIU Yao-ming | Institute of Applied Biology, Shanxi University, Taiyuan 030006, China | | | YU Zhi-tao | Institute of Applied Biology, Shanxi University, Taiyuan 030006, China | | | ZHU Wen-ya | Institute of Applied Biology, Shanxi University, Taiyuan 030006, China
Institute of Plant Protection, Shanxi Academy of Agriculture Science Research, Taiyuan 030032, China | | | LI Ya-hong | Institute of Applied Biology, Shanxi University, Taiyuan 030006, China | | | GUO Ya-ping | School of Life Science, Shanxi University, Taiyuan 030006, China
3 Shanxi Key Laboratory of Integrated Pest Management in Agriculture, Shanxi University, Taiyuan 030006, China | | | ZHANG Jian-zhen | Institute of Applied Biology, Shanxi University, Taiyuan 030006, China | | | WU Hai-hua | Institute of Applied Biology, Shanxi University, Taiyuan 030006, China | wuhaihua04@163.com | | MA En-bo | Institute of Applied Biology, Shanxi University, Taiyuan 030006, China | maenbo2003@sxu.edu.cn |
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| Abstract: |
| Oxya chinensis is an important agricultural pest and feeds mainly on rice. Adults of O. chinensis were treated with three heavy metals(CdCl2, CuCl2 and ZnSO4), respectively. Quantitative real-time PCR(qPCR) was used to measure OcMT1 and OcMT2 expressions in testis, ovary and muscle of O. chinensis. In the Cd treatments, mRNA levels of OcMT in three tissues were significantly up-regulated in a dose-dependent manner, compared to the control. However, the greatest expression of OcMT1 in testis and ovary was found at the lowest Cu concentration, while the expression of OcMT2 in three tissues increased significantly with Cu concentrations. Zinc treatments increased OcMT1 and OcMT2 expression levels in three tissues in a dose-dependent manner. These results show that the OcMT1 and OcMT2 in testis, ovary and muscle could be induced by three heavy metals, but their mRNA expression levels are different at different concentrations of heavy metals. OcMTs show different sensitivities to different heavy metals. The expressions and synthesis of MT induced by heavy metals would be of significance in protecting tissues by reducing toxicity of metals via binding with MT, which advances our understanding of how this insect deals with excessive heavy metals at molecular levels. |
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