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| Oxidative Stresses and DNA Damages in Cells of Vicia faba Exposed to Phenanthrene and Pyrene |
| Received:March 06, 2014 |
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| KeyWord:phenanthrene;pyrene;oxidative stress;DNA damage;comet assay |
| Author Name | Affiliation | E-mail | | GAO Xi | Institute of Organic Contaminant Control and Soil Remediation, Nanjing Agricultural University, Nanjing 210095, China | | | SHENG Yue-hui | Institute of Organic Contaminant Control and Soil Remediation, Nanjing Agricultural University, Nanjing 210095, China | | | GAO Yan-zheng | Institute of Organic Contaminant Control and Soil Remediation, Nanjing Agricultural University, Nanjing 210095, China | gaoyanzheng@njau.edu.cn |
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| Abstract: |
| Polycyclic aromatic hydrocarbons(PAHs) are persistent organic pollutants with toxic, carcinogenic, and mutagenic nature. Their influences on cells of higher plants have not been understood well. An experiment was conducted to investigate the oxidative stresses and DNA damages of plants induced by phenanthrene and pyrene. The relationship between DNA damages and oxidative stresses was also examined in the presence of the antioxidant Vitamin E. The MDA content and antioxidant enzyme activities(SOD, POD, and CAT) in Vicia faba were determined. The DNA damages were evaluated using the comet assay. Phenanthrene and pyrene enhanced the antioxidant enzyme activities and MDA contents. The content of MDA gradually increased with increasing concentrations of phenanthrene and pyrene. The values of tail moment(TM) from the comet assay increased from 46.41 μm(at the control) to 122.04 μm(at 50 mg·kg-1 phenanthrene treatment). Similarly, the degree of DNA damages aggravated with increasing pyrene concentrations(0~50 mg·kg-1). Pyrene at 50 mg·kg-1 caused significant(P<0.01) damages to the cell DNA of Vicia faba root tips and the TM value was 110.36 μm, 149.21% higher than the control(44.30 μm). The pretreatment of antioxidant vitamin E weakened the DNA damages of Vicia faba cells, suggesting that vitamin E could reduce DNA damages by phenanthrene and pyrene. In conclusion, phenanthrene and pyrene could cause oxidative stresses and DNA damages of Vicia faba, and that PAH-induced DNA damages are closely correlated with the oxidative stresses. |
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