|
| Acute Effects of Hg2+ and Cd2+ Pollution on Metallothionein in Hepatic Tissues of Lutjanus argentimaculatus |
| |
| View Full Text View/Add Comment Download reader |
| KeyWord:Lutjanus argentimaculatus; biomarker; metallothionein（MT）; mercury; cadmium |
| Author Name | Affiliation | | CHEN Chun-liang | Monitoring Center for Ocean Resources and Environments, Guangdong Ocean University, Zhanjiang 524088, China | | MA Sheng-wei | Key Lab of Fishery Ecology and Environment, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510300, China | | WANG Xue-feng | Fisheries College, Guangdong Ocean University
Zhanjiang 524088, China |
|
| Hits: 2029 |
| Download times: 2270 |
| Abstract: |
| Metallothionein（MT） plays important roles in binding and detoxifiying heavy metals. However, the responses of MT to acute pollution of Hg2+and Cd2+ in water have not been clear yet. In a semi-static experiment, the levels of MT in the hepatic tissues of Lutjanus argentimaculatus were determined with enzyme-linked binding assay（ELBA） under single and combined exposure to different concentrations of Hg2+ and Cd2+ at 3, 12, 24, 48, 96 h. Under exposure to single metal, the levels of MT were increased by 17.9%, 43.7% and 51.8% at 0.5, 1, 10 μg Hg·L-1, and by 4.3%, 54.7%, 84.9% at 5, 10, 100 μg Cd·L-1, respectively. Mixed exposure of Hg-Cd promoted MT levels by 22.8%，26.3%， 51.7% for Hg1Cd（0.5 μg·L-1 Hg2++10 μg·L-1 Cd2+），Hg2Cd（1 μg·L-1 Hg2++10 μg·L-1 Cd2+），Hg3Cd（10 μg·L-1 Hg2++10 μg·L-1 Cd2+）, and by 30.1%，26.4%， 58.3% for Cd1Hg（5 μg·L-1 Cd2++1 μg·L-1 Hg2+）, Cd2Hg（10 μg·L-1 Cd2++1 μg·L-1 Hg2+）, Cd3Hg（100 μg·L-1 Cd2++1 μg·L-1 Hg2+）, respectively. In conclusion, both single and mixed exposure of Hg2+ and Cd2+ increase MT levels in the hepatic tissues, with obvious time-response and dose-response. The induction of MT is lower in the mixed treatments than in their corresponding single ones, indicating the antagonism of Hg2+ and Cd2+. |
|
|
|
