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| Simultaneous Determination of Six Fluoroquinolones in Chicken Manures by High Performance Liquid Chromatography with Fluorescence Detection |
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| KeyWord:fluoroquinolones（FQs）; solid-phase extraction-high performance liquid chromatography with fluorescence detection; livestock and poultry excrement; analytical method |
| Author Name | Affiliation | | LIU Bo | College of Resources and Environment, Hunan Agricultural University, Changsha 410128, China
State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China | | XUE Nan-dong | State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China | | YANG Bing | State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China | | GONG Dao-xin | College of Resources and Environment, Hunan Agricultural University, Changsha 410128, China | | LI Fa-sheng | State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China | | ZHANG Shi-lei | State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China | | ZHOU Ling-li | State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China | | MENG Lei | State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China | | CHEN Xuan-yu | College of Resources and Environment, Hunan Agricultural University, Changsha 410128, China
State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China | | YAN Yun-zhong | State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China |
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| Abstract: |
| An analytical method for simultaneous determination of six fluoroquinolones（FQs） in chicken manures based on solid-phase extraction and high performance liquid chromatography with a fluorescence detection（HPLC/FLD） was developed in the current study. Samples were ultrasonically extracted in Mcllvaine-Na2EDTA buffer/acetonitrile（1∶1）（pH=2） for 15 min. The extract was cleaned by a HLB solid-phase extraction column, and then eluted with methanol solvent. The fluoroquinolones in the extract were determined by HPLC/FLD with acetonitrile/0.7% phosphate as mobile phase. In a concentration range of 0.01~1.0 mg·kg-1, R2 of calibration was greater than 0.99. The average percent recoveries ranged from 76.7% to 106.7% for six compounds in chicken manure samples at three different spiked levels（0.1, 0.5 mg·kg-1 and 1.0 mg·kg-1）. The detection limits（LOD） were from 0.002 mg·kg-1 to 0.022 mg·kg-1, and limit of quantification（LOQ） from 0.006 8 mg·kg-1 to 0.074 mg·kg-1. This method was used to analyze six FQs in chicken manures collected from 5 chicken farms in Beijing. All six FQs were detected in most samples, with concentrations ranging from LOD to 1.13 mg·kg-1. |
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