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| Effects of Biochar Applications on Bacterial Diversity in Fluvor-aquic Soil of North China |
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| KeyWord:biochar; fluvor-aquic soil; soil bacteria; biodiversity |
| Author Name | Affiliation | | WU Ying-ga | College of Life Science and Technology, Inner Mongolia Normal University, Hohhot 010022, China
Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | ZHANG Gui-long | Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | LAI Xin | Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | LIU Hong-mei | Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | YANG Dian-lin | Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China |
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| Abstract: |
| As a by-product of biomass pyrolysis, biochar has been deployed to alleviate anthropogenically triggered increases in atmospheric CO2 concentrations. It is generally accepted that biochar-C is largely unavailable to soil microbes but can change soil physicochemical properties. Applying biochar with metabolically available labile-C compounds may shift soil microbial community structure. In the present research, a field experiment was designed to investigate temporal changes in bacterial diversity after biochar additions in fluvor-aquic soil in North China. Six treatments with four replicates were used：CK1（no biochar or urea-N）; CK2（no biochar+225 kg urea-N·hm-2）; C1（7.5 t·hm-2 biochar +225 kg urea-N·hm-2）; C2（15 t·hm-2 biochar+225 kg urea-N·hm-2）; C3（25 t·hm-2 biochar+225 kg urea-N·hm-2）; C4（30 t·hm-2 biochar+225 kg urea-N·hm-2）. The biochar used in the experiment was pyrolytic peanut shell processed at 300 ℃. The experimental treatments were randomly assigned. Bacterial diversity was measured using DGGE-cloning sequencing method. Compared with controls（CK1, CK2）, biochar treatments increased DGGE fingerprints by 4 or 5 bands, but decreased the Shannon-Wiener diversity and Pielou evenness index by 11.5%~13.0%, 14.1%~16.5%, respectively. Cluster analysis indicated that DGGE fingerprints shared at least 60% similarity among all treatments. Additions of biochar reduced the similarity of bacterial community composition. Sequencing 13 bands selected from DGGE gel according to their peculiarity showed that new bands p, q and r were found but the bands e and g representing Proteobacteria disappeared in the treatment C3 and C4. The present results indicate that biochar promotes the growth of new bacteria but inhibit some known bacteria, thus changing the bacterial community composition and decreasing the diversity and evenness index of soil bacterial community. |
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