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Daphnid Biomass and Activity of Free-living NAGase Under Pesticide Stresses
  
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KeyWord:daphnids; chitobiase; biomass; pesticides
Author NameAffiliation
GUO Dan-dan Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou 310029, China 
LI Shao-nan Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou 310029, China 
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Abstract:
      Free β-N-acetyl-D-glucosaminidase(NAGase, or chitobiase) may be an indicator of aquatic arthropod with respect to pesticide stresses. In this experiment, three species of Daphnids, i.e. Daphnia magna Straus, Moina macrocopa, and Simocephalus vetulus and three presticides, lufenuron, diafenthiuron, and etoxazole, were used to study the arthropod biomass and free-living NAGase activity. It was found that arthropod biomass(x) and NAGase activity(y) had significant correlation(y=0.000 603x+0.004 33, r=0.687 and P=0.00), suggesting the feasibility of using free-living NAGase activity to predict the biomass of the exposed arthropod populations. In viro studies with 10 pesticides, i.e. lufenuron, diafenthiuron, and etoxazole, butachlor, trazodone, chlorpyrifos, fenvalerate, imidacloprid, pymetrozine, and chlorantraniliprole, showed that their minimal concentrations at which the activity of free-living NAGase was directly affected were much higher than the highest concentrations found in water bodies. These results suggest that the biomass could be predicted without underestimation by NAGase activity.