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Optimization of Conditions for Producing Reducing Sugar from Rice Straw Pretreated with Hydrochloric Acid
  
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KeyWord:rice straw; HCl pretreatment; total reducing sugar; orthogonal design; Box-Behnken design
Author NameAffiliation
CUI Mao-jin Department of Chemistry and Chemical Engineering, Henan Institute of Science and Technology, Xinxiang 453003, China 
HUANG Ming-xian Department of Chemistry and Chemical Engineering, Henan Institute of Science and Technology, Xinxiang 453003, China 
DENG Yue-e Department of Chemistry and Chemical Engineering, Henan Institute of Science and Technology, Xinxiang 453003, China 
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Abstract:
      Producing bioenergy from agricultural wastes(e.g. rice straw) is a way to kill two birds with one stone by recycling agricultural wastes and obtaining new energy. To improve saccharification efficiency of rice straw, orthogonal design and response surface methodology were used to optimize temperature, time and concentration of hydrochloric acid(HCl) pretreatment and rice straw rates. Box-Behnken design was then employed to further optimize the HCl pretreatment conditions. The factors influencing production of total reducing sugar were in order of temperature>HCl concentration>time>rice straw rates. The optimal conditions for HCl pretreatment of rice straw were temperature of 133.80 ℃, HCl concentration of 7.28%(W/V) and time of 51.12 min. The maximum concentration of total reducing sugar was 549.42 mg·g-1 under the optimal conditions, which was 6.01 times of that from raw materials. The results indicate that HCl pretreatment is necessary for higher saccharification of rice straw.