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| Acute Toxicity of Imidacloprid with Different Formulation on Earthworm and Zebrafish |
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| KeyWord:imidacloprid; formulation; earthworm（Eisenia foetida）; zebrafish; acute toxicity |
| Author Name | Affiliation | | CHEN Ai-mei | College of Resources and Environment, College of Plant Protection, Pesticide Environmental Toxicology Research Centre, Key Laboratory of Agricultural Environment in Universities of Shandong | | WANG Jin-hua | College of Resources and Environment, College of Plant Protection, Pesticide Environmental Toxicology Research Centre, Key Laboratory of Agricultural Environment in Universities of Shandong | | XIA Xiao-ming | College of Resources and Environment, College of Plant Protection, Pesticide Environmental Toxicology Research Centre, Key Laboratory of Agricultural Environment in Universities of Shandong | | WANG Juan | College of Resources and Environment, College of Plant Protection, Pesticide Environmental Toxicology Research Centre, Key Laboratory of Agricultural Environment in Universities of Shandong | | ZHU Lu-sheng | College of Resources and Environment, College of Plant Protection, Pesticide Environmental Toxicology Research Centre, Key Laboratory of Agricultural Environment in Universities of Shandong | | FAN Yan-yan | College of Environmental Science and Engineering, Yangzhou University |
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| Abstract: |
| An acute toxicity study was conducted to evaluate the ecological risk of pesticide with different formulations on environmental organisms by artificial soil test and static test. Results of artificial soil test showed that LC50 values of 35% imidacloprid aqueous suspension concentrate（SC）, 70% imidacloprid wet seed dressing agent（WS）, 70% imidacloprid wettable powder（WP）, and 600 g·L-1 imidacloprid flowable concentrate for seed coating（FS） for earthworm in 7 d were 0.82（0.62～0.89）, 6.44（5.17～7.83）, 2.39（2.10～2.61） and 9.65（7.79～11.77）a.i.mg·kg-1, respectively. Corresponding LC50 values in 14 d were 0.77（0.59～0.83）, 6.00（4.34～6.79）, 2.12（1.62～2.33） and 8.60（0.23～10.19） a.i.mg·kg-1, respectively. Results of a static test showed that the LC50 values of 70% imidacloprid WS for zebrafish on 24 h, 48 h, 72 h, and 96 h were 24.3（16.3～28.8）, 22.8（13.0～27.7）, 20.1（4.0～27.4） and 17.8（2.4～26.2） a.i.mg·L-1, respectively. LC50 values of 70% imidacloprid WP for zebrafish were 46.4（41.6～48.0）, 45.7（38.4～47.6）, 45.2（36.2～47.2） and 45.2（36.2～47.2） a.i.mg·L-1, respectively. LC50 values of both 35% imidacloprid SC and 600 g·L-1 imidacloprid FS for zebrafish were higher than 1.00×102 a.i.mg·L-1. According to the Experimental Criterion for Environmental Safety Assessment of Chemical Pesticide, the toxic effect of 35% imidacloprid SC on earthworms was significant, while other formulations of imidacloprid were moderate. In additional, the toxic effects of all different formulations of imidacloprid on zebrafish were not serious. |
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