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| Quantification of Swine-specific Bacteroidales in Water Using qPCR |
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| KeyWord:swine wastewater; Bacteroidales; biomarker; qPCR |
| Author Name | Affiliation | | WANG Xian-gui | Institute of Environment and Sustainable Development in Agriculture, CAAS, Beijing 100081, China | | GUO Ping | Institute of Environment and Sustainable Development in Agriculture, CAAS, Beijing 100081, China | | TIAN Yun-long | Institute of Environment and Sustainable Development in Agriculture, CAAS, Beijing 100081, China | | ZHU Chang-xiong | Institute of Environment and Sustainable Development in Agriculture, CAAS, Beijing 100081, China | | ZHANG Li | Institute of Environment and Sustainable Development in Agriculture, CAAS, Beijing 100081, China |
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| Abstract: |
| Application of Bacteroidales 16S rRNA gene markers in the assessment of water pollution by swine wastewater was examined with quantitative real time polymerase chain reaction（ｑＰＣＲ）. An improved cetyl trimethyl ammonium bromide（ＣＴＡＢ） method was established for extracting total DNA from swine wastewater. Primer 3（Bac32F/Bac108R） showed higher specificity and lower detection limit（4.09E+02~ 1.60E+03 copies） than others, and could be used to assess water pollution from swine wastewater. |
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