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| Isolation and Denitrification Characteristics of a Heterotrophic Nitrification Bacterium |
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| KeyWord:heterotrophic nitrification bacterium; isolation; Paracoccus denitrificans.; denitrification capacity |
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| Abstract: |
| The present paper dealt with isolation and denitrification characteristics of a heterotrophic nitrification bacterium, which isolated from the high NH3-N swine wastewater. Based on the screening methods published by some scientists, a isolating method in the study was put forward to used an acetamide as a sole carbon and nitrogen source to screen a heterotrophic nitrifying bacterium from the swine wastewater. An efficient heterotrophic nitrification bacterium was isolated and identified with 16S rDNA sequence. The phylogenetic analysis of the strain was performed based on the 16S rDNA sequence. The efficiency of heterotrophic nitrification was detected for the strain. The strain FJAT-14899 was identified to be Paracoccus denitrificans with the aid of 16S rDNA sequencing. The removal rates for the total concentrations of nitrogen and NH4-N were 81.7% and 53.7% in 12 h cultural time. The final removal rates of the total nitrogen and NH+4-N were 90.1% and 61.3% after 24 h culturing without accumulations of nitrite nitrogen and nitrate nitrogen. Paracoccus denitrificans FJAT-14899 had a higher removal rate of NH+4-N with promissing application in water treatment. |
