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| Effects of Phosphate Rock, Bone Char and Rape Straw on Quantity of Bacteria and Archaea in Soils Combined Polluted by Heavy Metals |
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| KeyWord:soils; heavy metal; amendments; bactera; archaea; real-time polymerase chain reaction |
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| Hits: 1936 |
| Download times: 1971 |
| Abstract: |
| The activity of microorganisms played an important role in soil ecosystem. As one of the sensitive indicators of monitoring the quality of soil, the gene copies numbers of microorganisms were more and more used to diagnose the change of soil environment. Effects of different amendments on the 16S rRNA gene copies numbers of bacteria and archaea in soil combined polluted by heavy metals were studied by using real-time polymerase chain reaction. The results showed that the range of 16S rRNA gene copies numbers of bacteria and archaea were 6.02×109~1.82×1010 and 7.99×106~2.97×107 copies per gram of dry soil, respectively. Addition of different amendments and planting maize could influence the 16S rRNA gene copies numbers of bacteria and archaea in soil contaminated by heavy metals. For the treatments without maize, addition of phosphate rock, bone char and rape straw led to the increase of gene copies of bacteria in soil by 116.8%~163.2% comparing with the control treatment, and the phosphate treatment and bone char treatment led to the increase of the gene copies of archaea in soil by 171.1% and 224.8% respectively. For the treatments planting maize, addition of bone char and rape straw led to the gene copies of bacteria in soil increase by 104.2% and 92.0% respectively comparing with the control treatment. Except for the gene copies of archaea in soil increase of 231.3% by adding bone char, there was no significant effect on the gene copies of archaea in soil by adding other amendments comparing with the control treatment. |
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