| 丁静,陈伟光,陈宇婷,何岸飞,姜晶,盛光遥.生物炭添加下枯草芽孢杆菌应对镉胁迫的分子机制[J].农业环境科学学报,2023,42(10):2324-2334. |
| 生物炭添加下枯草芽孢杆菌应对镉胁迫的分子机制 |
| Molecular mechanisms of Bacillus subtilis in response to cadmium stress under biochar addition |
| 投稿时间：2022-12-16 |
| DOI：10.11654/jaes.2022-1278 |
| 中文关键词: 生物炭 枯草芽孢杆菌 镉胁迫 转录组 分子机制 |
| 英文关键词: biochar Bacillus subtilis cadmium stress transcriptome molecular mechanism |
| 基金项目:江苏省自然科学基金项目（BK20180967）；城市生活污水资源化利用技术国家地方联合工程实验室（苏州科技大学）开放课题（2021KF05）；江苏省双创团队项目（2018—2017）；江苏省双创博士项目 |
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| 中文摘要: |
| 为了探究生物炭添加前后枯草芽孢杆菌应对镉胁迫的分子机制，本研究基于转录组测序，对比分析了枯草芽孢杆菌在4种体系（正常体系、镉胁迫体系、生物炭体系、生物炭-镉胁迫体系）中的基因表达差异。结果表明：在镉胁迫压力下，枯草芽孢杆菌共产生1 932个差异表达基因，其中上调基因967个，下调基因965个。枯草芽孢杆菌的代谢活性受到明显抑制，但是其鞭毛组装和细菌趋化性通路明显增强，离子转运和阳离子跨膜转运蛋白功能条目的基因表达也呈现上调，说明枯草芽孢杆菌通过上调细胞运动及离子转运相关基因的表达来规避镉毒性压力。相比于镉胁迫体系，在生物炭-镉胁迫体系中，细菌共产生1 541个差异表达基因，其中上调基因691个，下调基因850个。生物炭的存在增强了枯草芽孢杆菌生物过程、细胞组分和分子功能基因的表达，枯草芽孢杆菌物质和能源代谢相关通路的基因表达均呈现上调，并且细菌体内镉抗性基因czcD、cadA和膜功能相关差异基因的表达也以上调为主，说明生物炭可以缓解镉胁迫对枯草芽孢杆菌的毒性效应，并可刺激枯草芽孢杆菌的金属抗性能力。 |
| 英文摘要: |
| To explore the molecular mechanism of Bacillus subtilis in response to cadmium stress before and after biochar addition, based on transcriptome sequencing, this study compared and analyzed the differences in gene expression of B. subtilis in four systems(normal, cadmium, biochar, and biochar-cadmium system). The results revealed that there were 1 932 differentially expressed genes in B. subtilis under cadmium stress, including 967 up-regulated genes and 965 down-regulated genes. The metabolic activity of B. subtilis was significantly inhibited. However, its flagellum assembly and bacterial chemotaxis pathway were significantly enhanced. The gene expressions of ion transport and cationic transmembrane transporter functional items were up-regulated, suggesting that B. subtilis avoids cadmium toxic pressure by up-regulating the expression of genes related to cell movement and ion transport. Compared with the cadmium system, 1 541 differentially expressed genes were produced in the biochar-cadmium system, including 691 up-regulated and 850 downregulated genes. The presence of biochar enhanced the expression of bioprocess, cell components, and molecular function genes in B. subtilis. The gene expressions related to substance and energy metabolism in B. subtilis were up-regulated. The expression of the cadmium resistance gene czcD and cadA and the membrane function-related differential gene in B. subtilis was mainly regulated. These results indicated that biochar could alleviate the toxic effect of cadmium stress on B. subtilis and stimulate the metal resistance of B. subtilis. |
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