| .镉和铬急性染毒对中华稻蝗解毒酶及多酚氧化酶的影响[J].农业环境科学学报,2013,32(7):. |
| 镉和铬急性染毒对中华稻蝗解毒酶及多酚氧化酶的影响 |
| Acute Effects of Cd2+ and Cr6+ on Detoxification Enzymes and Polyphenol Oxidase in Oxya chinensis |
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| DOI：10.11654/jaes.2013.07.006 |
| 中文关键词: 镉 铬 代谢酶 多酚氧化酶 中华稻蝗 |
| 英文关键词: Cd Cr detoxification enzyme polyphenol oxidase Oxya chinensis |
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| 摘要点击次数: 1960 |
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| 中文摘要: |
| 为了解重金属对生物体营养物质的消化、吸收、代谢以及对昆虫的毒性效应，研究了重金属镉和铬急性染毒对中华稻蝗5龄若虫几种代谢解毒酶及多酚氧化酶活性的影响。研究结果显示：高浓度镉和铬分别在48 h和72 h激活CarE活性；当镉处理中华稻蝗96 h后，体内ACP活性下降；铬染毒72 h后，AKP活性升高，而铬作用24 h和48 h后，ACP活性表现为在低浓度处理组中被激活，高浓度处理组中被抑制；镉处理48 h后，GST活性升高，铬处理72 h和96 h，GST有升高的趋势；镉处理24 h和铬处理96 h后PPO活性降低。这些结果表明，镉和铬染毒后，中华稻蝗体内代谢解毒酶及多酚氧化酶活性发生变化，这种变化与重金属的浓度及作用时间密切相关。 |
| 英文摘要: |
| Acute effects of Cd2+ and Cr6+ on detoxification enzymes and polyphenol oxidase（CarE, GST, ACP, AKP and PPO） in 5th instar nymphs of Oxya chinensis were studied under laboratory control condition. The results showed that CarE activities were activated after insects were exposed to the highest concentration of Cd2+（7.08 mg·kg-1） for 48 h and Cr6+（3.40 mg·kg-1） for 72 h, respectively. ACP activity was inhibited by Cd2+ at 96 h. AKP activity increased-in exposed to Cr6+ for 72 h. However, ACP activity increased at the lower concentration of 1.80 mg·kg-1 Cr6+ and declined at the higher concentration of 3.40 mg·kg-1 Cr6+ at 24 h and 48 h. GST activites were increased by Cd2+ at 48 h and by Cr6+ at 72 h and 96 h, respectively. PPO activities were decreased by Cd2+ at 24 h and by Cr6+ at 96 h, respectively. These results indicated that detoxification enzymes and polyphenol oxidase activity changed in response to toxic effects of heavy metal（Cd and Cr）, while the changes were related with the exposed dose and time of heavy metal. |
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