| .戊二醛胁迫对黑藻光合作用及抗氧化酶活性的影响[J].农业环境科学学报,2013,32(6):. |
| 戊二醛胁迫对黑藻光合作用及抗氧化酶活性的影响 |
| Effects of Glutaraldehyde Stress on Photosynthesis and Antioxidase Activity of Hydrilla Verticillata |
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| DOI：10.11654/jaes.2013.06.009 |
| 中文关键词: 戊二醛 黑藻 抗氧化酶活性 光合速率 |
| 英文关键词: glutaraldehyde Hydrilla verticillata antioxidase activity photosynthetic rate |
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| 中文摘要: |
| 为探讨戊二醛对常见沉水植物的危害，用含有0、1、5、10、50 mg·L-1戊二醛的1/20 Hoagland营养液培养沉水植物黑藻，就不同浓度的戊二醛胁迫下黑藻叶绿素含量、光合速率、抗氧化酶（SOD、POD和CAT）活性和MDA含量变化进行试验研究。结果表明：戊二醛抑制叶绿素的合成或使其分解加速，叶绿素含量下降，光合速率下降；1、5 mg·L-1的戊二醛处理1 d和3 d后，SOD活性上升；1、5、10 mg·L-1的戊二醛处理1 d和3 d后，POD活性上升；经1 mg·L-1戊二醛处理后，CAT活性均处于较高水平，但当戊二醛浓度大于等于5 mg·L-1时，CAT活性与对照相比明显下降。经1 mg·L-1戊二醛处理，MDA含量没有明显变化；经5、10、50 mg·L-1戊二醛处理3 d后，MDA含量上升。低浓度戊二醛胁迫后能诱导黑藻的抗氧化酶活性增加，随着胁迫处理时间的延长和处理浓度的增大，抗氧化酶活性下降，但1 mg·L-1戊二醛处理7 d后，CAT活性没有下降，说明CAT对戊二醛的耐受能力较强。 |
| 英文摘要: |
| In order to evaluate the injury and inherent physiological mechanism of glutaraldehyde stress on advanced aquatic organisms, Hydrilla verticillata was cultivated in 1/20 Hoagland solution with 0 mg·L-1, 1 mg·L-1, 5 mg·L-1, 10 and 50 mg·L-1 glutaraldehyde. Chlorophyll content, photosynthetic rate, antioxidase（SOD, POD and CAT） activity and MDA content were measured. The results showed that with the increase of concentration of glutaraldehyde and the extension of stress time, the content of chlorophyll a and chlorophyll b declined and the photosynthetic rate dropped. Under the treatment of 1 mg·L-1 or 5 mg·L-1 glutaraldehyde for 1 day and 3 days, SOD activity increased. But at 10 mg·L-1 or higher concentration of glutaraldehyde, it was significantly lower than that of the control. POD activity increased significantly under the treatment of 1 mg·L-1, 5 mg·L-1 and 10 mg·L-1 glutaraldehyde for 1 day and 3 days, but when the stress time extended to 5 days, it showed an absolute dropping even at low concentration of 1 mg·L-1 glutaraldehyde. At a general level, during all treatments, CAT activity showed a higher level under 1 mg·L-1 glutaraldehyde, but 5 mg·L-1 or higher concentration of glutaraldehyde stress made CAT activity lower than that of the control. MDA content remained almost unchanged in 1 day stress of all glutaraldehyde concentrations. However, when the stress time prolonged to 3 days and longer, it showed a rising tendency as the glutaraldehyde concentration increased. Generally, the results showed that antioxidase activity of H. verticillata was stimulated by low concentration of glutaradehyde but declined with higher glutaradehyde concentration and prolonging stress time. It′s worth noting that CAT activity did not decline with the treatment of 1 mg·L-1 glutaraldehyde for 7 days, suggesting CAT might be more tolerant to low content of glutaraldehyde than SOD and POD. |
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