| 庄杰,孙乐妮,杨恩东,欧阳文凯,曹媛媛,张夕红.金属抗性促生细菌Burkholderia sp.减缓玉米幼苗镉毒害和阻抗镉转运[J].农业环境科学学报,2024,43(4):763-773. |
| 金属抗性促生细菌Burkholderia sp.减缓玉米幼苗镉毒害和阻抗镉转运 |
| Alleviation of Cd toxicity and impedance of Cd transport in maize seedlings by the metal resistant-plant growth promoting bacterium Burkholderia sp. |
| 投稿时间：2023-09-02 |
| DOI：10.11654/jaes.2023-0711 |
| 中文关键词: 金属抗性促生细菌 玉米 镉 转运基因 生理特性 |
| 英文关键词: metal resistant and plant growth promoting bacteria maize Cd transport gene physiological trait |
| 基金项目:安徽省高校自然科学研究项目（KJ2021A0169）；安徽省自然科学基金项目（2008085MD120） |
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| 中文摘要: |
| 为探明金属抗性促生细菌对减缓玉米镉毒害和阻抗镉转运的影响，本研究采用砂培试验向营养液中添加不同浓度重金属镉，研究接种镉抗性促生细菌YM3（Burkholderia sp.）对玉米苗干质量、生理指标、镉含量及金属转运基因表达情况的影响。结果表明，接种菌株YM3能促进玉米生长，且在镉胁迫环境中菌株促生效果更显著，使玉米根、茎、叶干质量分别增加9.09%~40.00%、63.33%~84.41%、48.50%~67.48%；接种处理玉米叶绿素总量增加1.70%~53.17%、根系活力增加7.40%~16.93%；游离脯氨酸降低3.04%~21.82%；当镉浓度为12 mg·L-1时，丙二醛含量降低26.37%；接种菌株显著降低玉米地上部镉含量，使茎、叶镉含量分别降低13.64%~41.84%和17.85%~20.29%；q-PCR对金属转运相关基因HMA2、HMA3和Nramp5进行表达量的检测，结果表明接种菌株对重金属转运相关基因转录水平的表达调控受镉浓度影响。在0 mg·L-1和8 mg·L-1镉处理中，接菌处理HMA2、HMA3和Nramp5表达量在根中均表现为下调，在8 mg·L-1和12 mg·L-1镉处理中，接菌处理HMA2和Nramp5表达量在叶中均表现为下调。接菌处理通过不同程度影响基因HMA2、HMA3和Nramp5表达量来阻控玉米苗对镉的吸收转运。研究表明，在镉胁迫环境下接种镉抗性促生菌株Burkholderia sp.YM3能促进玉米苗生长，减缓玉米苗镉毒害，并阻控镉从根部向地上部转运，这对于玉米安全生产具有重要应用潜力。 |
| 英文摘要: |
| A sand culture experiment was conducted to investigate how metal-resistant and plant growth-promoting bacterium alleviate Cd toxicity and reduce Cd transport in maize. Different concentrations of Cd were added to the nutrient solution to assess how inoculation with Cd resistant and plant growth promoting bacterium YM3（Burkholderia sp.）affect the dry weight, physiological indicators, Cd content, and metal transport gene expression in maize seedlings. The results showed that inoculation with the bacterial strain YM3 could promote maize growth. Moreover, this growth promoting effect was more pronounced under Cd stress, resulting in an increase in dry weight of maize roots, stems, and leaves by 9.09%-40.00%, 63.33%-84.41%, and 48.50%-67.48%, respectively. Total chlorophyll content and root activity of maize increased by 1.70%-53.17% and 7.40%-16.93%, respectively, after inoculation. Free proline decreased by 3.04%-21.82%. At a Cd concentration of 12 mg·L-1, malondialdehyde content decreased by 26.37%. Inoculation of the YM3 strain significantly reduced the Cd content of the aboveground parts of maize, resulting in a reduction in Cd content of 13.64%-41.84% for stems and leaves and 17.85%- 20.29% for leaves. q-PCR was used to detect the expression levels of metal transport related genes HMA2, HMA3, and Nramp5. The results showed that the YM3-regulated expression of heavy metal transport related genes was influenced by Cd concentration. In the inoculated 0 and 8 mg·L-1 Cd treatments, the expression levels of HMA2, HMA3, and Nramp5 in the roots were down regulated. In the inoculated 8 and 12 mg·L-1 Cd treatments, the expression levels of HMA2 and Nramp5 in the leaves were also down regulated. The inoculation treatment inhibited the absorption and transportation of Cd in maize seedlings by varying degrees, affecting the expression levels of the genes HMA2, HMA3 and Nramp5. In summary, inoculation with Cd resistant and plant growth promoting Burkholderia sp. strain YM3 under Cd stress can promote the growth of maize seedlings, alleviate Cd toxicity in maize seedlings, and prevent Cd transport from roots to aboveground parts, which has the potential to promote safe production of maize. |
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