| 杨玲,张艺,钟俊杰,聂三安,孙耿,盛浩.不同调酸剂对种植玉米红壤微生物群落的影响[J].农业环境科学学报,2024,43(3):609-616. |
| 不同调酸剂对种植玉米红壤微生物群落的影响 |
| Effects of different acid modulators on the microbial communities in maize planting red soil |
| 投稿时间：2023-05-16 |
| DOI：10.11654/jaes.2023-0386 |
| 中文关键词: 红壤 调酸 宏基因组 微生物群落 碳/氮代谢过程 |
| 英文关键词: red soil acid regulation metagenome microbial community carbon/nitrogen metabolism |
| 基金项目:国家自然科学基金项目（42177288，41907088）；湖南省自然科学基金项目（2023JJ30307）；国家自然科学基金联合项目（U19A2048） |
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| 中文摘要: |
| 为研究红壤微生物丰度和群落组成对不同调酸剂的响应，分析影响碳/氮关键代谢过程微生物的变化，通过盆栽实验，设置不施肥（CK）、钙镁复合剂（L）、钙镁复合剂配施猪粪（ML）和钙镁复合剂配施秸秆（SL）4个处理，采用宏基因组测序技术，分析土壤细菌、真菌和古菌以及碳/氮代谢关键过程微生物。结果表明：L、ML和SL处理显著提高土壤pH值和交换性钙/镁，显著降低土壤交换性酸。调酸剂增加了细菌优势菌中的变形菌门相对丰度，降低了绿弯菌门和酸杆菌门相对丰度；降低了真菌优势菌中的毛霉菌门相对丰度；增加了古菌优势菌中的广古菌门和深古菌门的相对丰度，降低了奇古菌门的相对丰度。冗余分析结果显示，速效钾是影响土壤细菌和真菌群落结构的主要环境因子，土壤pH和有机碳是影响土壤真菌和古菌群落结构组成的关键因子。碳代谢过程的贡献度方面，变形菌门的贡献度在SL处理中最高，放线菌门和芽单胞菌门的贡献度在ML处理中最高。氮代谢过程中，各处理绿弯菌门对硝化作用的贡献率均超过80%。调酸降低了绿弯菌门和酸杆菌门在反硝化与硝酸盐异化还原过程中的贡献度，L与SL处理的变形菌门贡献度低于ML处理，而ML处理的放线菌门贡献度高于L与SL处理。单施钙镁复合剂、钙镁复合剂配施猪粪和钙镁复合剂配施秸秆均可缓解红壤酸性，改变土壤优势菌群，并有利于碳/氮积累。 |
| 英文摘要: |
| The aims of this study were to investigate the response of red soil microbial abundance and community composition to different acid regulators as well as the changes in microorganisms that affect key carbon/nitrogen metabolic processes. Four potted treatments were set up:no fertilization(CK), calcium/magnesium compound(L), L mixed with pig manure(ML), and L mixed with straw(SL). Metagenomic sequencing technology was used to analyze the soil microbes and key carbon/nitrogen metabolism process microorganisms. The results showed that the L, ML, and SL treatments significantly increased the soil pH and exchangeable calcium and magnesium, but significantly reduced the soil exchangeable acids. The acid regulators increased the bacterial relative abundance of Proteobacteria but decreased Chloroflexi and Acidobacteria while reducing the fungal relative abundance of Mucoromycota. The archaeal relative abundance of Euryarchaeota and Candidatus_Bathyarchaeota was increased, while Thaumarchaeota was decreased. The RDA analysis showed that available potassium is the main factor that affects the structure of soil bacterial and fungal communities, while soil pH and organic carbon are key factors that affect the composition of soil fungal and archaeal communities. In terms of the carbon metabolism process, Proteobacteria had the highest contribution in the SL treatment, while in the ML the highest contribution was attributed to Actinobacteria and Gemmatimonadetes. In the process of nitrogen metabolism, Chloroflexi contributed more than 80% toward nitrification. Acid regulation reduced the contribution of Chloroflexi and Acidobacteria in the process of denitrification and nitrate dissimilation reduction. The contribution of Proteobacteria to SL was lower than that to ML, while the contribution of Actinobacteria to ML was higher than that to L and SL. The application of L, ML, and SL may alleviate the acidity of red soil, change the dominant microbial community, and facilitate carbon/nitrogen accumulation. |
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