| 曾贤良,方汉孙,王润萍,隗黎丽,阮记明,李福贵,熊六凤,高瑾,梁惜梅.甲苯咪唑对大鳞副泥鳅肝脏氧化损伤的毒性作用[J].农业环境科学学报,2024,43(2):271-277. |
| 甲苯咪唑对大鳞副泥鳅肝脏氧化损伤的毒性作用 |
| Oxidative damage of mebendazole to the liver of Paramisgurnus dabryanus |
| 投稿时间：2023-06-15 |
| DOI：10.11654/jaes.2023-0471 |
| 中文关键词: 甲苯咪唑 大鳞副泥鳅 抗氧化系统 酶活性 |
| 英文关键词: mebendazole Paramisgurnus dabryanus antioxidant system enzyme activity |
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| 中文摘要: |
| 为了探究甲苯咪唑对大鳞副泥鳅肝脏氧化损伤的毒性作用，将大鳞副泥鳅暴露于浓度为0 （对照）、0.004、0.02、0.1 mg·L-1的甲苯咪唑溶液中，分别于24、72、144 h测定大鳞副泥鳅肝脏谷丙转氨酶（GPT）、谷草转氨酶（GOT）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）、乙酰胆碱酯酶（AChE）活性和总抗氧化能力（T-AOC）及丙二醛（MDA）含量的变化。结果表明： 24 h时GOT活性随着甲苯咪唑浓度的增加呈下降趋势，72 h和144 h时则呈先升高后下降趋势；低（0.004 mg·L-1）、中（0.02 mg·L-1）和高（0.1 mg·L-1）浓度组GPT活性在甲苯咪唑整个暴露过程中均低于对照组； SOD活性除了中浓度组在72 h时显著高于对照组外（P<0.05），各浓度组SOD活性整体上呈现出不同程度的抑制趋势；在甲苯咪唑的整个暴露过程中，GPx活性和T-AOC水平均受到显著抑制（P<0.05），而MDA含量受到不同程度的诱导； 24 h时各浓度组中AChE活性均显著低于对照组（P<0.05），72 h和144 h时AChE活性则随着甲苯咪唑暴露浓度的增加而升高，低浓度组受到显著抑制（P<0.05），而高浓度组受到显著诱导（P<0.05）。研究表明，甲苯咪唑暴露可对大鳞副泥鳅肝脏组织产生氧化胁迫和损伤作用，干扰神经递质系统，并对鱼类机体产生神经毒性效应。 |
| 英文摘要: |
| To explore the toxic effects of mebendazole via oxidative damage to the liver of Paramisgurnus dabryanus, P. dabryanus were exposed to mebendazole solution concentrations of 0（control）, 0.004, 0.02 mg·L-1, and 0.1 mg·L-1. The activities of glutamic pyruvic transaminase（GPT）, glutamic oxaloacetic transaminase（GOT）, superoxide dismutase（SOD）, glutathione peroxidase（GPx）, and acetylcholinesterase（AChE）; the total antioxidant capacity（T-AOC）; and malondialdehyde（MDA） content in the liver tissue of P. dabryanus were measured at 24, 72 h, and 144 h. The results showed that the activity of GOT decreased with the increase in mebendazole concentration at 24 h, but increased and then decreased at 72 h and 144 h, respectively. The activity of GPT in the low（0.004 mg·L-1）, medium（0.02 mg·L-1）, and high（0.1 mg·L-1）concentration groups was lower than that in the control group during the entire experiment. The SOD activity in all concentration groups showed a trend of inhibition, except for the medium concentration group at 72 h. The GPx activity and T-AOC level were significantly inhibited（P<0.05）during the entire exposure to mebendazole, whereas the MDA content was induced to varying degrees. Furthermore, at 24 h, the AChE activity in each concentration group was significantly lower than that in the control group（P<0.05）, whereas at 72 h and 144 h, the AChE activity increased with the increase in mebendazole concentration; it was also significantly suppressed in the low concentration group（P<0.05）and significantly induced in the high concentration group（P<0.05）. These results suggest that exposure to mebendazole can cause oxidative stress and damage to the liver tissue of P. dabryanus, interfere with the neurotransmitter system, and produce neurotoxic effects in fish. |
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