| 俞诗音,潘淑桢,唐敏,王美仙.‘光谱’月季对Cd、Zn的富集特征和耐性机制[J].农业环境科学学报,2024,43(1):48-59. |
| ‘光谱’月季对Cd、Zn的富集特征和耐性机制 |
| Cd and Zn accumulation characteristics and tolerance mechanism of Rosa ‘Spectra’ |
| 投稿时间：2023-03-14 |
| DOI：10.11654/jaes.2023-0186 |
| 中文关键词: ‘光谱’月季 Cd Zn 富集特征 耐性机制 |
| 英文关键词: Rosa ‘Spectra’ Cd Zn accumulation characteristics tolerance mechanism |
| 基金项目:国家自然科学基金项目（31600574）；北京市共建项目专项；北京林业大学建设世界一流学科和特色发展引导专项资金项目（2019XKJS0322）；北京市教委科学研究与研究生培养共建科研项目（2019GJ-03） |
| 作者 | 单位 | E-mail | | 俞诗音 | 北京林业大学园林学院, 国家花卉工程技术研究中心, 城乡生态环境北京实验室, 北京 100083 | | | 潘淑桢 | 北京林业大学园林学院, 国家花卉工程技术研究中心, 城乡生态环境北京实验室, 北京 100083 | | | 唐敏 | 北京林业大学园林学院, 国家花卉工程技术研究中心, 城乡生态环境北京实验室, 北京 100083 | | | 王美仙 | 北京林业大学园林学院, 国家花卉工程技术研究中心, 城乡生态环境北京实验室, 北京 100083 | wangmx@bjfu.edu.cn |
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| 中文摘要: |
| 为进一步研究月季对 Cd、Zn 的富集特征和耐性机制，以‘光谱’月季（Rosa‘Spectra’）为研究对象，采用盆栽污染模拟试验，研究不同Cd含量（0、25、50、100、200 mg·kg-1）和Zn含量（0、500、1 000、1 500、2 000 mg·kg-1）单一胁迫下‘光谱’月季的生物量和生理指标变化，以及重金属积累转运机制。结果表明：低含量Cd、Zn处理促进‘光谱’月季的生长，随着处理含量的增加，‘光谱’月季生长受到抑制，但都未致死。‘光谱’月季体内吸收的Cd、Zn主要积累在根系，含量分别为68.48、918.74 mg·kg-1，其中Cd主要以 NaCl 提取态存在，占比为 25.19%~60.80%，Zn 主要以 HAc 提取态和去离子水提取态存在，占比分别为 20.20%~46.65% 和12.69%~29.33%。随处理含量的增加，Cd、Zn处理组都提高了毒性较低的提取态占比，以减弱Cd、Zn对植物根系的毒害。根系中的 Cd、Zn均主要富集在细胞碎屑组分，占比分别为 55.01%~77.38%、52.35%~63.17%，其次是金属富集颗粒组分和热稳定蛋白组分。随处理含量的增加，‘光谱’月季根系内的Cd、Zn表现出向可溶性组分转移的特征。Cd、Zn处理下，‘光谱’月季根系细胞中均出现黑色颗粒物质，且其密度随着处理含量的增加而增大。根系细胞壁上与Cd、Zn结合的官能团均是羟基、氨基。研究表明，通过根部吸收限制、细胞壁固定、液泡区隔化将Cd、Zn转化为低活性的形态赋存，以及用细胞壁上的羟基、氨基提供结合位点，可能是‘光谱’月季应对Cd、Zn胁迫的重要机制。 |
| 英文摘要: |
| Simulated pot pollution was adopted with Rosa ‘Spectra’ as the research object to further study the enrichment characteristics and tolerance mechanisms of Cd and Zn in Chinese rose. The biomass and physiological indexes, as well as the mechanism of heavy metal accumulation and transport of Rosa ‘Spectra’ were studied under the single stress of Cd content（0, 25, 50, 100 mg·kg-1, and 200 mg·kg-1） and Zn content（0, 500, 1 000, 1 500 mg ·kg-1, and 2 000 mg ·kg-1）. The results showed that：the treatment of low Cd and Zn content promoted the growth of Rosa ‘Spectra’ . With an increase in content, the growth of Rosa ‘Spectra’ was inhibited, but no death was caused. Cd and Zn absorbed by the Rosa ‘Spectra’ were mainly accumulated in the root system, with contents of 68.48 mg·kg-1 and 918.74 mg·kg-1 respectively. Cd predominately existed in NaCl- extracted forms, accounting for 25.19%-60.80%, while Zn primarily existed in HAc-extracted and deionized water forms, accounting for 20.20% - 46.65%, 12.69% - 29.33% respectively. With the increase of treatment gradients, both Cd and Zn treatment groups increased the proportion of extraction states with low toxicity, so as to weaken the toxicity of Cd and Zn in plant roots. Cd and Zn enrichment in the roots were mostly in the cellular debris fraction, accounting for 55.01% - 77.38%, 52.35% - 63.17% respectively, and followed by the metal-rich granule and heat stable protein fractions. With the increase in treatment gradients, Cd and Zn in the root of Rosa ‘Spectra’ showed characteristics of transferring to soluble components. Under Cd and Zn treatment, black particulate matter appeared in root cells of Rosa ‘Spectra’, and the density increased with increasing treatment gradients. The functional groups binding Cd and Zn on the root cell wall are hydroxyl and amino groups. Research shows that root absorption restriction, cell wall fixation, vacuoles segregation, conversion of Cd and Zn into less active forms, and the use of hydroxyl and amino groups on the cell wall to provide binding sites may be significant mechanisms for Rosa ‘Spectra’ to cope with Cd and Zn stress. |
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