| 何建勇,覃惠松,蒋代华,黄雪娇,李春秀,王怡桢,黄智刚,朱正杰.叶面喷施微生物合成纳米硒对水稻硒镉吸收的影响[J].农业环境科学学报,2023,42(11):2398-2409. |
| 叶面喷施微生物合成纳米硒对水稻硒镉吸收的影响 |
| Effects of nano-selenium synthesized by microorganisms sprayed on leaf surfaces on cadmium selenide absorption in rice |
| 投稿时间：2023-05-12 |
| DOI：10.11654/jaes.2023-0372 |
| 中文关键词: 微生物合成纳米硒 硒 镉 无机硒 有机硒 水稻 |
| 英文关键词: microbial synthesis of nano-Se selenium cadmium inorganic selenium organic selenium rice |
| 基金项目:国家自然科学地区合作与交流项目(41967006)；广西自然科学基金重点项目(2018GXNSFDA281035)；巴马县人才科技计划项目(20220031)；广西农业环境与农产品安全重点实验室2023年度开放基金(GKLAEAPS2023-05) |
| 作者 | 单位 | E-mail | | 何建勇 | 广西大学农学院, 广西农业环境与农产品安全重点实验室, 南宁 530000 | | | 覃惠松 | 广西大学农学院, 广西农业环境与农产品安全重点实验室, 南宁 530000 | | | 蒋代华 | 广西大学农学院, 广西农业环境与农产品安全重点实验室, 南宁 530000 | dhjiang2008@gxu.edu.cn | | 黄雪娇 | 广西大学农学院, 广西农业环境与农产品安全重点实验室, 南宁 530000 | | | 李春秀 | 广西大学农学院, 广西农业环境与农产品安全重点实验室, 南宁 530000 | | | 王怡桢 | 广西大学农学院, 广西农业环境与农产品安全重点实验室, 南宁 530000 | | | 黄智刚 | 广西大学农学院, 广西农业环境与农产品安全重点实验室, 南宁 530000 | | | 朱正杰 | 百色学院, 广西 百色 533000 | |
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| 中文摘要: |
| 为探讨喷施微生物合成纳米Se(B)对水稻Se、Cd吸收的影响与籽粒富Se降Cd的效果,本研究通过盆栽试验,研究微生物合成SeNPs喷施5(B5)、10(B10)、20(B20) mg·L-1对水稻Se、Cd吸收、转运效应,以及与无机Se(S)、有机Se(M)喷施对比,比较水稻籽粒富Se降Cd效果。结果表明,与不喷Se(CK)、S、M处理相比,B5、B10、B20处理提高籽粒Se含量分别为227.52%~366.46%、3.38%~23.31%、30.54%~42.57%,降低籽粒Cd含量分别为10.14%~55.07%、4.08%~36.37%、9.03%~40.77%,籽粒富Se降Cd效果表现为B>S>M>CK,B处理中,籽粒富Se降Cd效果以喷施20 mg·L-1浓度最佳,高Se与低Se水稻品种间对Se、Cd吸收差异不显著。与CK处理相比,B处理促进了水稻叶片中Cd在细胞壁、液泡组分中的积累,减少了叶绿体、线粒体组分中的积累,促进了叶片中Se在细胞壁、叶绿体组分中的积累,减少了线粒体、液泡组分中的积累。主成分分析和Pearson相关矩阵表明,水稻各部位Se与Cd含量呈负相关关系(P ≤ 0.01)。与CK、M处理相比,B处理对水稻Se生物富集系数分别提高20.63%~264.70%、20.37%~32.86%,表现为S>B>M>CK,B5、B10、B20处理对水稻Cd富集系数分别降低9.10%~25.10%、5.70%~9.65%、10.64%~22.10%,表现为CK>M>S>B。供试水稻Se转移系数表现为根-茎<茎-籽粒<茎-叶,Cd转移系数表现为根-茎<茎-叶<茎-籽粒,与CK、S、M处理相比,B处理均促进了Se向籽粒的转移,减少了Cd向籽粒的富集。与CK、S、M处理相比,B处理均显著提高了水稻叶片SOD、CAT、POD、APX、PAL、Pro、GSH含量,降低了MDA、H2O2的含量,高Se水稻品种(泰丰优208)对提高水稻抗氧化酶活性与抗氧化性物质含量效果高于低Se水稻品种(桂野丰)。因此,叶面喷施微生物合成SeNPs在调控水稻Se、Cd吸收、转运与籽粒富Se降Cd方面有着更显著的优势。 |
| 英文摘要: |
| This study aimed to investigate the effects of microbial synthesis of nano-Se(B) on the absorption of cadmium selenide in rice and grain enrichment of Se on Cd reduction. The effects of microbial synthesis SeNPs sprayed with 5(B5), 10(B10), and 20(B20) mg·L-1 on the absorption and transport of cadmium selenide in rice were studied through a pot experiment. Further, the effects of Se enrichment and Cd reduction on rice grains with inorganic Se(S) and organic Se(M) spray were compared. Compared with no Se(CK), S, and M treatments, B5, B10, B20 treatment increased Se content by 227.52%-366.46%, 3.38%-23.31%, and 30.54%-42.57%, and decreased Cd content by 10.14%-55.07%, 4.08%-36.37%, and 9.03%-40.77%, respectively. The effect of Se enrichment on Cd reduction was in the order B>S>M>CK. In B treatment, the best effect of Se enrichment on Cd reduction was observed with a 20 mg·L-1 spray. Further, no significant difference in Se and Cd absorption between high-and low-Se rice varieties was observed. Compared with CK treatment, B treatment promoted the accumulation of Cd in cell wall and vacuole components, reduced the accumulation of chloroplast and mitochondrial components, promoted the accumulation of Se in cell wall and chloroplast components, and reduced the accumulation of mitochondria and vacuole components in rice leaves. The principal component analysis and Pearson correlation matrix showed a negative correlation between Se and Cd content in each part of rice (P ≤ 0.01). Compared with CK, M treatments, the bio-enrichment coefficients of Se in B treatment were increased by 20.63%-264.70%, 20.37%-32.86%, respectively, in the order S>B>M>CK. CK and B5, B10, B20 treatments reduced the Cd enrichment coefficients of rice by 9.10%-25.10%, 5.70%-9.65%, and 10.64%-22.10%, respectively, in the order CK>M>S>B. The Se transfer coefficient of rice was in the order root-stem2O2. The effect of high-Se rice varieties(Tai Fengyou 208) on improving the activity of antioxidant enzymes and the content of antioxidant substances in rice leaves was higher than that of low-Se rice varieties(Gui Yefeng). Therefore, SeNPs synthesized by microorganisms sprayed on the leaf surface have more significant advantages in regulating the absorption and transport of Se and Cd in rice, enriching Se, and reducing Cd in grain. |
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