| 张冰,王玖程,汪冬至,冯建骋,侯文峰,周雪,高强.施用三环唑与二氯喹啉酸对水稻土细菌的影响[J].农业环境科学学报,2023,42(7):1514-1521. |
| 施用三环唑与二氯喹啉酸对水稻土细菌的影响 |
| Effects of tricyclazole and quinclorac acid on bacteria in paddy field soil |
| 投稿时间：2022-12-01 |
| DOI：10.11654/jaes.2022-1235 |
| 中文关键词: 群落多样性 群落结构 水稻土 三环唑 二氯喹啉酸 |
| 英文关键词: community diversity community structure paddy soil tricyclazole quinclorac acid |
| 基金项目:国家自然科学基金项目（41807146）；吉林省自然科学基金项目（20190201274JC）；吉林省教育厅“十三五”科学技术项目（JJKH20190908KJ） |
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| 中文摘要: |
| 为探究三环唑与二氯喹啉酸对稻田土壤细菌多样性和群落结构的影响，2021年在吉林省吉林市永吉县布设田间试验，利用高通量测序技术，研究推荐施用量（低剂量）和2倍推荐施用量（高剂量）对稻田土壤细菌群落的影响。结果表明：高剂量或低剂量的三环唑与二氯喹啉酸对细菌α多样性均无显著影响（P>0.05），但对细菌群落结构产生显著影响（P<0.05），且高剂量施用的影响大于低剂量；门水平注释结果显示，丰度前 5 的菌门为 Proteobacteria（丰度占比为 33.54%）、Chloroflexi（20.75%）、Acidobacteria（13.81%）、Bacteroidetes（9.86%）、Actinobacteria（8.48%），高剂量施用对菌门相对丰度的影响大于低剂量；对丰富类群和稀有类群的分析发现，施用不同剂量的三环唑和二氯喹啉酸均造成稀有类群减少，条件稀有类群增加；特化种分析结构显示，不同处理对共有特化种所属菌门丰度均造成影响，普遍规律为高剂量施用的影响大于低剂量施用，与二氯喹啉酸相比，三环唑的施用产生了更多的特化种，且其所属菌门分布更广泛，其中高剂量施用三环唑处理产生的特化种所属菌门数最多（6个），低剂量施用三环唑处理和低剂量施用二氯喹啉酸处理各产生1个。综上所述，施用三环唑与二氯喹啉酸不会造成稻田土壤细菌多样性的显著改变，但显著影响了细菌的群落结构及物种组成，且高剂量施用影响大于低剂量施用。在实际应用中，三环唑与二氯喹啉酸的施用在满足生产所需的前提下应选择推荐施用的低剂量，以减少对土壤微生物群落的潜在不利影响。 |
| 英文摘要: |
| To investigate the effects of tricyclazole and quinclorac acid application on soil bacterial diversity and community structure in rice fields, a field trial was set up in Yongji County, Jilin City, Jilin Province in 2021. High-throughput sequencing technology was used to investigate the effects of the recommended application rate（low dose）and two times the recommended application rate（high dose）on the soil bacterial community in rice fields. Both high and low dose applications of tricyclazole and quinclorac acid had no significant effect on bacterial α diversity（P>0.05）, but they had a significant effect on bacterial community structure（P<0.05）, and the effect of high dose application was greater than that of low dose application. The phylum annotation results showed that the most abundant phylum was Proteobacteria, with the highest abundance ratio of 33.54%, followed by Chloroflexi（20.75%）and Acidobacteria（13.81%）. The top five phyla were completed by bacteroidetes, which accounted for 9.86% and Actinobacteria, which accounted for 8.48%. The effect of high dose application on the relative abundance of bacteroidetes was greater than that of low dose application. Analyzing abundant and rare taxa showed that applying different doses of tricyclazole and quinclorac acid caused a decrease in rare taxa and an increase in conditionally rare taxa, without affecting the remaining taxa. The structure of the specialized species analysis showed that the different treatments affected the abundance of the phylum distributed by the shared specialized species. The general pattern was that the high dose application had a greater effect than the low dose application, and triconazole application produced more specialized species and their distributed endemic phyla than quinclorac acid application. There were a maximum of six in the high-dose triconazole treatment and one each in the low-dose triconazole treatment and the low-dose quinclorac acid treatment. In summary, although triconazole and quinclorac acid application did not cause significant changes in soil bacteria diversity in rice fields, it significantly affected community structure and species composition. The effect of high-dose application was greater than that of low-dose application, indicating that triconazole and quinclorac acid application in practice should follow the recommended low dose to meet the needs of production and the impact of pesticides on the soil environment and ecological safety. |
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