| 王秀君,朱文博,朱天娇,张秋萍,庞小可,许继飞.氨氮对牛粪厌氧消化中四环素类抗性基因丰度及其驱动因子的影响[J].农业环境科学学报,2023,42(5):1139-1147. |
| 氨氮对牛粪厌氧消化中四环素类抗性基因丰度及其驱动因子的影响 |
| Effects of ammonia nitrogen on the abundance of tetracycline resistance genes and its driving factors during anaerobic digestion of dairy manure |
| 投稿时间：2022-09-17 |
| DOI：10.11654/jaes.2022-0918 |
| 中文关键词: 四环素类抗性基因 牛粪 厌氧消化 氨氮浓度 水平基因转移 |
| 英文关键词: tetracycline resistance genes dairy manure thermophilic anaerobic digestion concentrations of ammonia nitrogen horizontal gene transfer |
| 基金项目:内蒙古自然科学基金项目（2020MS05003）；内蒙古自治区科技计划项目（2020GG0015，2020GG0081）；国家自然科学基金项目（51768048） |
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| 中文摘要: |
| 为探究氨氮浓度对高温厌氧消化中四环素类抗生素抗性基因（Tetracycline resistance genes，TRGs）绝对丰度变化及其微生态机制，设置氨氮浓度为600、1 100 mg·L-1和1 600 mg·L-1的牛粪高温厌氧消化体系，分析了TRGs、可移动遗传元件和细菌群落结构的变化特征及三者之间的关系。结果表明：氨氮浓度为600 mg·L-1和1 100 mg·L-1时，高温厌氧消化的产气速率和总产气量相似；氨氮浓度为1 600 mg·L-1时，二者均受到抑制。tetC、tetO、tetQ、tetT和tetX的丰度在不同氨氮浓度条件下均减少，但tetA和tetG的丰度在氨氮浓度为1 600 mg·L-1条件下增加了1.05倍和1.85倍。不同处理中细菌群落差异明显，TRGs潜在宿主菌的种类和数目均改变。相关性分析表明TRGs潜在宿主菌的差异可一定程度解释TRGs的丰度变化，但tetA和tetG丰度在氨氮浓度为1 600mg·L-1条件下的增加主要与intI1和intI2的增长有关。综上所述，牛粪中较高的氨氮浓度会增加高温厌氧消化过程中TRGs通过水平基因转移途径增殖的可能性，从而增加TRGs传播的风险。 |
| 英文摘要: |
| To reveal the effects of different concentrations of ammonia nitrogen(600, 1 100 mg·L-1 and 1 600 mg·L-1) on the changes in the abundances of tetracycline resistance genes(TRGs) during thermophilic anaerobic digestion(TAD) of dairy manure, the variations of TRGs, mobile genetic elements, and bacterial community were compared and analyzed. The results showed that biogas production rates and total biogas yields were similar among TAD with ammonia nitrogen concentrations of 600 mg·L-1 and 1 100 mg·L-1, but they were inhibited in TAD under an ammonia nitrogen concentration of 1 600 mg·L-1. tetC、tetO、tetQ、tetT 和 tetX reduced in all TADs. However, tetA and tetG increased by 1.05 times and 1.85 times after TAD with an ammonia nitrogen concentration of 1 600 mg·L-1. Differences in bacterial communities were observed among three treatments. The kind and number of potential host bacteria of TRGs changed as well. Correlation analysis demonstrated that the differences in potential host bacteria of TRGs could be the reason for the changes of TRGs at a certain level; however, the increase in tetA and tetG was related to the increase in intI1 and intI2. In summary, high concentrations of ammonia and nitrogen in dairy manure might enhance the horizontal gene transfer of TRGs during TADs, leading to an increased risk of the spread of TRGs. |
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