| 余果,孙丽娜,唐蕊,韩悦.全氟丁烷磺酸对其耐受菌胞外聚合物特征的影响[J].农业环境科学学报,2023,42(5):1032-1041. |
| 全氟丁烷磺酸对其耐受菌胞外聚合物特征的影响 |
| Effects of perfluorobutanesulfonic acid on the extracellular polymeric substances of PFBS-resistant strains |
| 投稿时间：2022-09-25 |
| DOI：10.11654/jaes.2022-0947 |
| 中文关键词: 全氟丁烷磺酸 胞外聚合物 三维荧光光谱 红外光谱 高效液相色谱-质谱联用 |
| 英文关键词: perfluorobutanesulfonic acid extracellular polymeric substances three-dimensional fluorescence spectrum infrared spectroscopy high performance liquid chromatography-tandem mass spectrometry |
| 基金项目:国家重点基础研究发展计划（973计划）项目（2014CB441106） |
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| 中文摘要: |
| 为确定在全氟丁烷磺酸（PFBS）胁迫下两种耐受菌胞外聚合物（EPS）的组分变化特征，从阜新某氟化工污水处理厂污泥中分离出两种PFBS耐受菌株，通过生长量（OD600）和代谢活性（ETSA）测定、16S rDNA序列系统发育树分析、总有机碳测定、PFBS去除率测定、三维荧光光谱和红外光谱分析，研究了100 μg·L-1 PFBS胁迫下两菌株的生长量与代谢活性，EPS产量、组成特征、官能团的变化及其对PFBS的去除作用。结果表明：这两种耐受菌被鉴定为Pseudomonas（假单胞菌属）和Serratia（沙雷氏菌属），它们能在100 μg·L-1 PFBS胁迫下正常生长，且对PFBS均有去除效果，去除率分别为33.18%和19.95%，在100 μg·L-1 PFBS胁迫下，两种耐受菌的EPS产量、蛋白质含量、多糖含量均降低，而蛋白质/多糖比值增加，EPS的蛋白峰（色氨酸和酪氨酸）、腐植酸和富里酸强度均降低，C-O、O-H和N-H等官能团峰强度降低。研究表明100 μg·L-1 PFBS对两种耐受菌株的EPS组分有抑制作用。 |
| 英文摘要: |
| To determine the characterization of the component changes in extracellular polymers(EPS) of two tolerant strains under perfluorobutane sulfonic acid(PFBS) stress, two PFBS-tolerant strains were isolated from fluorochemical wastewater treatment plant sludge in Fuxin. Through growth optical density(OD600) and electron transport system activity(ETSA) determination, tree analysis, total organic carbon determination, PFBS removal rate determination, three-dimensional fluorescence spectroscopy and infrared spectroscopy analysis, we studied their growth and metabolic activity, EPS production, compositional characteristics, changes in functional groups and removal of PFBS under 100 μg·L-1 PFBS stress. The study showed that two identified strains, Pseudomonas sp. and Serratia sp., were able to grow under 100 μg·L-1 PFBS stress, and were effective in removing PFBS, with the removal rates of 33.18% and 19.95% respectively. When the PFBS concentration is 100 μg·L-1, EPS concentration, protein content, and polysaccharide content of the two strains decreased, and the protein/polysaccharide ratio increased. The protein peaks(tryptophan and tyrosine) and humic acid and fulvic acid intensity of EPS decreased, and the intensity of functional groups such as C-O, O-H and N-H decreased. The above results indicate that 100 μg·L-1 PFBS inhibited the EPS components of the two resistant strains. |
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