| 于广泉,魏孝承,王倩,王强,张春雪,杨波,郑向群.人尿施用对生菜品质性状及土壤细菌群落结构的影响[J].农业环境科学学报,2023,42(3):558-567. |
| 人尿施用对生菜品质性状及土壤细菌群落结构的影响 |
| Effects of human urine application on lettuce quality traits and soil bacterial community structure |
| 投稿时间：2022-08-10 |
| DOI：10.11654/jaes.2022-0811 |
| 中文关键词: 人尿|细菌群落结构|生菜|品质性状 |
| 英文关键词: human urine|bacteria community structure|lettuce|quality trait |
| 基金项目:中央级公益性科研院所基本科研业务费专项（Y2022LM13）；创新工程所级重点项目（2022-cxgczdrw-zxq） |
|
| 摘要点击次数: 891 |
| 全文下载次数: 833 |
| 中文摘要: |
| 人尿作为一种传统的有机液体肥料，具有广泛的应用前景。为探讨人尿施用对作物品质性状和土壤微生物群落结构的影响，本研究以温室大棚盆栽生菜（Lactuca sativa L.）为研究对象，设置人尿0 mL·kg-1（CK）、人尿13 mL·kg-1（T1）、人尿26 mL·kg-1（T2）和人尿52 mL·kg-1（T3）4种不同施肥管理的盆栽试验，测定生菜的产量及品质性状，并对土壤采用高通量测序技术探究不同人尿施用量对土壤细菌群落结构的影响。结果表明：与CK处理相比，T1、T2处理的生菜鲜质量分别提高了56.75%和100.01%，可溶性糖含量分别上升了90.63%、210.94%，但Vc含量分别降低了13.93%、26.62%，且T3处理生菜无法生长；与CK相比，施用尿液（T1~T3）使土壤TN、TP、AP、AK、NH+4-N、SOM含量分别上升了2.22%~37.78%、7.94%~20.63%、22.42%~128.90%、7.71%~83.70%、84.62%~159.62%、3.64%~7.52%，同时也使土壤EC提高了13.56%~161.02%，土壤C/N和pH分别下降了2.77%~21.90%和2.10%~10.28%；随着人尿施用量的增加，OTUs总数不断降低，Shannon指数不断降低，Simpson指数不断升高，土壤变形菌门（Proteobacteria）和芽单胞菌门（Gemmatimonadetes）的相对丰度逐渐增加，而厚壁菌门（Firmicutes）的相对丰度逐渐下降；另外，各处理对放线菌门（Actinobacteria）、绿弯菌门（Chloroflexi）和酸杆菌门（Acidobacteria）等优势菌门的相对丰度均呈先升高后降低的趋势，其中T1处理达到最高值，较CK分别升高了14.71%、2.06%和16.17%。综上所述，在一定范围内，施用人尿能够显著增加生菜的鲜质量和可溶性糖含量，提高土壤养分含量，但过量施用会影响土壤细菌群落结构，降低细菌群落的多样性和丰度，生菜也无法正常生长。 |
| 英文摘要: |
| Human urine, as a traditional organic liquid fertilizer, has wide application prospects. To investigate the effects of human urine application on soil microbial community structure, we conducted a potted lettuce（Lactuca sativa L.）experiment in a greenhouse with four different fertilizer applications：0 mL·kg-1（CK）, 13 mL·kg-1（T1）, 26 mL·kg-1（T2）and 52 mL·kg-1（T3）human urine. We measured the yield and quality traits of lettuce in these treatments. The results showed that compared with CK, the fresh weight of T1 and T2 lettuce increased 56.75% and 100.01%, respectively; and the soluble sugar content increased by 90.63% and 210.94%, respectively; but the Vc content decreased by 13.93% and 26.62%, respectively. Moreover, in T3, the lettuce did not grow. Compared with CK, the application of urine（T1-T3）increased soil TN, TP, AP, AK, NH+4-N, and SOM contents by 2.22%-37.78%, 7.94%-20.63%, 22.42%-128.90%, 7.71%-83.70%, 84.62%-159.62%, and 3.64%-7.52%, respectively, but also increased soil EC by 13.56%-161.02%; soil C/N and pH decreased by 2.77%-21.90% and 2.10%-10.28%. With the increase in human urine dosage, the total number of OTUs decreased, the Shannon index decreased, and the Simpson index increased. With the increase in human urine dosage, the relative abundance of Proteobacteria and Gemmatimonadetes in the soil gradually increased, while the relative abundance of Firmicutes gradually decreased, and the relative abundance of Actinobacteria, Chloroflexi and Acidobacteria in each treatment showed a trend of increasing and then decreasing. We obtained the highest values from T1 lettuce, where they were increased by 14.71%, 2.06%, and 16.17%, respectively, compared with those of CK. In summary, within a certain range, the application of human urine can substantially increase the fresh weight and soluble sugar content of lettuce, and improve the soil nutrient content, but excessive application affects the structure of the soil bacterial community and reduces the diversity and abundance of the bacterial community, and thus lettuce does not grow normally. |
| HTML
查看全文
查看/发表评论 下载PDF阅读器 |
|
|
|
